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Non-invasive diagnosis of non-melanoma skin cancer subtypes predicted by microRNAs recovered from adhesive tape discs

ABSTRACT: We investigated whether a tape-stripping approach could be used for analysis of microRNA (miR) expression as a non-invasive method for prediction of non-melanoma skin cancer subtypes. From direct miR sequencing of RNA recovered from adhesive tape discs applied to skin lesions prior to biopsy, we determined rank lists of miRs to predict superficial or invasive BCC or SCC. Expression of these miRs was then assessed by digital PCR of disc-derived miR from independent validation cohorts of BCC (n=24 Sup, n=38 Inv) and SCC (n=45 SCC-in-situ, n=14 Inv). Expression ratios or products of >2 miRs were superior to individual miRs in discriminating Sup from Inv BCC and SCC subtypes. Moreover, several miR combinations distinguished Sup BCC and Inv SCC from a cohort of benign keratoses that can clinically resemble these cancers.

ORGANISM(S): Homo sapiens

PROVIDER: GSE286566 | GEO | 2025/01/20

REPOSITORIES: GEO

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