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Non-invasive diagnosis of non-melanoma skin cancer subtypes predicted by microRNAs recovered from adhesive tape discs

ABSTRACT: Non-invasive diagnosis of non-melanoma skin cancer subtypes predicted by microRNAs recovered from adhesive tape discs

PROVIDER: PRJNA1209927 | ENA |

REPOSITORIES: ENA

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Non-invasive diagnosis of non-melanoma skin cancer subtypes predicted by microRNAs recovered from adhesive tape discs

Project description:We investigated whether a tape-stripping approach could be used for analysis of microRNA (miR) expression as a non-invasive method for prediction of non-melanoma skin cancer subtypes. From direct miR sequencing of RNA recovered from adhesive tape discs applied to skin lesions prior to biopsy, we determined rank lists of miRs to predict superficial or invasive BCC or SCC. Expression of these miRs was then assessed by digital PCR of disc-derived miR from independent validation cohorts of BCC (n=24 Sup, n=38 Inv) and SCC (n=45 SCC-in-situ, n=14 Inv). Expression ratios or products of >2 miRs were superior to individual miRs in discriminating Sup from Inv BCC and SCC subtypes. Moreover, several miR combinations distinguished Sup BCC and Inv SCC from a cohort of benign keratoses that can clinically resemble these cancers.

2025-01-20 | GSE286566 | GEO

RNA-sequencing analysis of SD rat abdominal wall treated with adhesive or non-adhesive implant

Project description:We utilize bulk RNA-seq to profile the mRNA expression in day 3 and day 14 post-implantation of SD rat abdminal wall treated with adhesive or non-adhesive implants

2023-06-01 | GSE198219 | GEO

Comparison of Tape-Stripping Platforms as Non-Invasive Solutions for Skin Biomarker Discovery

Project description:Use of tape stripping for the collection of stratum corneum is a versatile and reliable procedure for the evaluation of the human skin health, including assessments of the skin barrier function, microbial content, and disease biomarkers. The tape stripping procedure is widely referenced; however, the number and type of tapes required for sample collection vary considerably, and the lack of standardized sampling, processing and normalization protocols complicate data comparison and interpretation. We have compared the performances of two commonly used non-invasive skin sampling platforms by collecting skin tissue from 34 healthy volunteers and applying standardization throughout the workflow, from sampling to data analysis. Our results showed significant differences in the amounts of tissue collected per tape, erythema levels, RNA and protein yields. RNA extracts from both platforms were suitable for sequencing; comparable input and quality of reads were produced for both test groups, however significant differences were found in the percentage of uniquely mapped reads and detected genes. This data highlights processing optimization as an important step in maximizing the efficiency of biomolecule extraction from tape strips and further presents a tape stripping method suitable for fast, efficient, and non-invasive epidermal tissue collection applicable to skin biomarker discovery.

2024-04-24 | GSE262827 | GEO

RNA-sequencing analysis of SD rat abdominal wall treated with adhesive or non-adhesive implant

Project description:RNA-sequencing analysis of SD rat abdominal wall treated with adhesive or non-adhesive implant

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The stratum corneum transcriptome in atopic dermatitis can be assessed by tape stripping

Project description:Background: Skin biopsies represent a gold standard in skin immunology and pathology but can cause pain and induce scarring. Non-invasive techniques will facilitate study recruitment of e.g. patients with pediatric atopic dermatitis (AD), hand eczema or facial dermatitis. Objective: By RNA sequencing, we examined whether the stratum corneum transcriptome in AD skin can be assessed by tape stripping, as compared to the epidermal transcriptome of AD in skin biopsies. To make the procedure clinically relevant tape strips were stored and shipped at room temperature for up to 3 days. Methods: Nine adult Caucasian AD patients and three healthy volunteers were included. Tape samples were collected from non-lesional and lesional skin. Biopsies were collected from lesional skin and were split into epidermis and dermis. Total RNA was extracted, and shotgun sequencing was performed. Results: Shotgun sequencing could be performed on skin cells obtained from two consecutive tape strips which had been stored and shipped at room temperature for up to three days. The most prominent differences between the tape strip and biopsy derived transcriptome were due to structural genes, while established molecular markers of AD, including CCL17, CCL22, IL17A and S100A7-S100A9, were also identified in tape strip samples. Furthermore, the tape strip derived transcriptome showed promise in also analysing the skin microbiome. Conclusion: Our study shows that the stratum corneum (SC) transcriptome of AD can be assessed by tape stripping the skin, supporting that this method may be central in future skin biomarker research.

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Proteomic analysis of tape-stripped actininc keratosis

Project description:This project aims to evaluates Tape Stripping (TS) of stratum corneum (SC) for non-invasive proteomic analysis of AK using mass spectrometry approach.

2018-10-01 | PXD011000 | Pride

The proteome of hand eczema assessed by tape stripping

Project description:Hand eczema (HE) is a prevalent skin disease. However, classification of HE into different subtypes remains challenging. Limited number of prior studies have employed invasive biopsy-based strategies; yet, studies of the HE proteome using non-invasive tape stripping methodology have not been reported. In this study, we wanted to assess whether global proteomic analysis of skin tape strip samples can be used for sub-classification of HE patients. Tape strips were collected from patients with HE and healthy skin. Liquid chromatography–mass spectrometry (LC/MS) proteomics was performed, and the global protein expression was analyzed. We identified 2,919 proteins in stratum corneum-derived skin cells from tape strip samples. Compared to healthy skin, the lesional samples from HE patients exhibited increased expression of immune-related markers and a decreased expression of structural barrier proteins. The difference between HE subtypes was restricted to the lesional skin areas, and included an increased expression of skin barrier-related proteins independently of the concurrent AD. In conclusion we found, that the non-invasive tape strip method used in combination with LC/MS proteomics can be used for analysis of skin protein expression in HE patients. Thus, the method shows potential for assessing the proteomic differences between subtypes of HE, and biomarker discovery.

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Non-invasive tape-stripping of psoriatic skin with high-resolution RNA profiling

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