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Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage in Darr strains expressing ArrWT, ArrH18A, ArrD83A, or an empty vector vs WT M. smegmatis Mc2155 (mgm182, site(+))

ABSTRACT: Transcriptional profiling of Mycobacterium smegmatis comparing strains undergoing I-SceI generated DNA damage at a single genomic locus Gene designations are the updated annotation

ORGANISM(S): Mycolicibacterium smegmatis

PROVIDER: GSE30635 | GEO | 2011/07/15

SECONDARY ACCESSION(S): PRJNA144627

REPOSITORIES: GEO

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Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage (site(+), mgm182) vs control (site(-), mgm181)

2011-07-15 | GSE30633 | GEO

Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage in Darr strains expressing ArrWT, ArrH18A, ArrD83A, or an empty vector vs WT M. smegmatis Mc2155 (mgm182, site(+))

Project description:Transcriptional profiling of Mycobacterium smegmatis comparing strains undergoing I-SceI generated DNA damage at a single genomic locus Gene designations are the updated annotation Four comparisons were made, all with log phase cultures and no ATc added. Darr Site(+) with an empty vector compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing WT Arr compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing ArrH18A compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing Arr D83A compared to wild-type M. smegmatis Site(+). 3 biological replicates of each strain for each experiment.

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Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage (site(+), mgm182) vs control (site(-), mgm181)

Project description:Transcriptional profiling of Mycobacterium smegmatis comparing a strain undergoing I-SceI generated DNA damage at a single genomic locus versus a control strain with no I-SceI recognition sequence in its genome (and thus not undergoing double strand DNA breaks Gene designations are from the original annotation, not the updated Five conditions investigated: Log phase cultures without induction of I-SceI expression (T=0, -Atc), Log phase cultures induced to express I-SceI by the addition of ATc for 45 minutes (T=45, +ATc), Stationary cultures control without induction of I-SceI expression (T=0, -Atc), Stationary phase cultures induced to express I-SceI by the addition of ATc for 15 minutes (T=15, +ATc), and Stationary cultures induced to express I-SceI for 45 minutes by the addition of ATc (T=45, +ATc). 3 biological replicates of each strain for each experiment.

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