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Expression data from Ad-β-catenin-GFP or Ad-GFP infected cardiomyocytes

ABSTRACT: The Wnt pathway is a key regulator of embryonic development, cell growth, differentiation, polarity formation, neural development, carcinogenesis, and stem cell self-renewal, and deregulation of the Wnt signalling is associated with many human disease. The central player in the Wnt pathway is β-catenin, A recent study has shown that β-catenin/Tcf/Lef signaling pathway is an essential growth-regulatory pathway in cardiomyocytes. We used DNA microarrays to detail the global trends in gene expression underlying β-catenin-overexpressed cardiomyocytes and identified distinct classes of up- or down-regulated genes during this process. Our findings suggest that β-catenin plays a critical role in regulating cardiac dysfunction at transcriptional level and may provide novel insight into how β-catenin modulates heart diseases.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE34772 | GEO | 2011/12/30

SECONDARY ACCESSION(S): PRJNA150345

REPOSITORIES: GEO

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Expression data from Ad-β-catenin-GFP or Ad-GFP infected cardiomyocytes

Project description:The Wnt pathway is a key regulator of embryonic development, cell growth, differentiation, polarity formation, neural development, carcinogenesis, and stem cell self-renewal, and deregulation of the Wnt signalling is associated with many human disease. The central player in the Wnt pathway is β-catenin, A recent study has shown that β-catenin/Tcf/Lef signaling pathway is an essential growth-regulatory pathway in cardiomyocytes. We used DNA microarrays to detail the global trends in gene expression underlying β-catenin-overexpressed cardiomyocytes and identified distinct classes of up- or down-regulated genes during this process. Our findings suggest that β-catenin plays a critical role in regulating cardiac dysfunction at transcriptional level and may provide novel insight into how β-catenin modulates heart diseases. Cardiomyocytes were infected with GFP control or β-catenin adenoviruses for RNA extraction and hybridization on Affymetrix microarrays. We sought to define the effects of β-catenin on the global programme of gene expression in primary cardiomyocytes. To that end, neonatal rat cardiomyocytes were infected with GFP control (G) or β-catenin adenovirus (B) for 24 hours.

2011-12-30 | E-GEOD-34772 | biostudies-arrayexpress

TCF/LEF dependent and independent regulation of Wnt/beta-catenin transcription

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TCF/LEF dependent and independent regulation of Wnt/beta-catenin transcription

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Project description:The forkhead box transcription factor FOXQ1 is aberrantly induced in various cancers, and contributes to tumour growth and metastasis. It has been suggested that the oncogenic potential of FOXQ1 may be explained by its activation of the Wnt/β-catenin signalling pathway. However, the mode of action of FOXQ1 in the Wnt pathway remains to be resolved. Here we report that FOXQ1 is bimodal transcriptional regulator of Wnt target gene expression in normal and cancer cells. Using co-immunoprecipitation, proximity proteomics, and reporter assays, we show that FOXQ1 partly engages the Wnt transcriptional complex to promote gene expression via TCF/LEF transcription factors.

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Cell-line-specific gene expression changes following inactivation of the CTNNB1 gene in human colorectal cancer cell lines

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Project description:The canonical Wnt signaling pathway is mediated by interaction of b-catenin with the TCF/LEF transcription factors and subsequent transcriptional activation of Wnt-target genes. In the hematopoietic system, the function of the pathway has been mainly investigated by genetic manipulation of b-catenin. However, this approach does not allow to discriminate between TCF/LEF dependent or independent b-catenin activity. Here, we employed a transgenic mouse model expressing a truncated dominant negative form of the human TCF4 transcription factor (dnTCF4) which abrogates activation of Wnt target genes even when b-catenin is stabilized and translocated into the nucleus while preserving its TCF/LEF independent activities. The disruption of the b-catenin-TCF/LEF interaction results in reduced granulocytic differentiation accompanied with an accumulation of short-term hematopoietic stem cells (ST-HSC) and granulocytic progenitors. In order to investigate the mechanism responsible for the insufficient production of granulocytes, we performed a transcriptomic analysis of ST-HSC from dnTCF4-expressing and control WT mice.

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ChIP-chip from mouse keratinocytes with Plakoglobin and β-catenin

Project description:Plakoglobin (PG; γ-Catenin, JUP) is a protein with controversial function. First described as a component of intercellular junctions, it has remained unresolved whether this near-ubiquitously expressed protein acts as a genuine transcriptional regulator in adult tissue like its closest relative β-catenin. Here we have mapped the global gene targets of PG by ChIP-chip in differentiating skin keratinocytes. Applying a peak algorithm, over 5’000 high-confidence PG target promoters were identified and 2’000 for β-catenin with an overlap of 38%. Bioinformatics analyses most significantly associated PG target genes with the Wnt signaling pathway as well as relevant pathways for keratinocyte differentiation. Using a combination of wild-type, PG, β-catenin and PG/β-catenin double null keratinocytes, PG was functionally validated as a LEF/TCF-dependent transcriptional regulator. These data challenge the current understanding of Wnt signaling, one of the most important pathways in tissue homeostasis, by identifying PG as a potent LEF/TCF-dependent transcriptional regulator which functionally overlaps with β-catenin.

2017-12-01 | GSE37343 | GEO

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