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Genes regulated by Sox9

ABSTRACT: We compared gene expression profile of jejunum crypt samples of Sox9 deficient mice and wild type control mice in order to identify the genes that are regulated by SOX9 in the small intestinal crypt epithelial cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE35481 | GEO | 2012/03/23

SECONDARY ACCESSION(S): PRJNA152369

REPOSITORIES: GEO

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Genes regulated by Sox9

Project description:We compared gene expression profile of jejunum crypt samples of Sox9 deficient mice and wild type control mice in order to identify the genes that are regulated by SOX9 in the small intestinal crypt epithelial cells. The intestinal epithelial cells were collected from crypts of jejunum of three 3-mo-old Sox9 mutant mice and three littermate controls.

2012-03-23 | E-GEOD-35481 | biostudies-arrayexpress

Comparison of Sox9-EGFP cell type transcriptomes during irradiation-induced crypt regeneration

2012-06-17 | E-GEOD-32227 | biostudies-arrayexpress

Comparison of Sox9-EGFP cell type transcriptomes during irradiation-induced crypt regeneration

2012-06-18 | GSE32227 | GEO

Characterization of IGF1 effects on the transcriptome of normal and irradiated Sox9-EGFP cell populations

2015-04-14 | GSE42817 | GEO

Characterization of IGF1 effects on the transcriptome of normal and irradiated Sox9-EGFP cell populations

Project description:Transcriptional profiling of intestinal epithelial cells expressing either Negative, Sublow, Low or High levels of the Sox9-EGFP reporter transgene FACS-isolated from jejunum of non-irradiated mice or at 5 days after 14Gy abdominal irradiation. In both groups, mice were treated for 5 consecutive days with either IGF1 or vehicle via mini-pumps (Alzet 1007D, IGF1 at 10mg/ml) implanted subcutaneously immediately following radiation. 4 distinct cell populations FACS-isolated based on levels of expression of the Sox9-EGFP reporter transgene (Sox9-EGFP Negative, Sublow, Low and High cells). 4 conditions: Non-irradiated/vehicle vs Non-irradiated/IGF1 vs Irradiated/vehicle vs Irradiated/IGF1. Biological replicates: 7 independent non-irradiated mice treated with vehicle - 3 independent non-irradiated mice treated for 5 days with IGF1 - 3 independent irradiated mice studied at day 5 post-irradiation treated with vehicle - 3 independent irradiated mice studied at day 5 post-irradiation treated for 5 days with IGF1.

2015-04-14 | E-GEOD-42817 | biostudies-arrayexpress

Essential role of Arid1a in intestinal stem cell maintenance and homeostasis through Sox9 regulation (ChIP-Seq)

Project description:Arid1a, a subunit of the SWI/SNF chromatin remodeling complex, have been reported in multiple human cancers, however, its functional role in intestinal homeostasis remains unclear. To investigate the role of Arid1a in murine intestine, we have employed ChIP experiments in intestinal spheroid cells generated from crypt cells of wild type mice. ChIP assay revealed that Arid1a binds directly to the Sox9 promoter to support its expression.

2018-10-24 | GSE121658 | GEO

Robust Three-dimensional (3D) Expansion of Bovine Intestinal Stem Cells: A Model for Replacement of Animal Experiments Toward Animal Welfare

Project description:we successfully developed bovine intestinal organoids representing typical crypt-villus and epithelium’s characteristics from bovine small intestinal (jejunum) crypts

2021-07-28 | GSE163425 | GEO

Profiling of Sox9-related genes in chondrogenesis using Sox9-3'EGFP knock-in and Sox9-EGFP/EGFP null chimeric mouse embryos

Project description:To define the repertoire of Sox9-dependent genes that contribute to the regulation of chondrogenesis, we generated Sox9-3'enhanced green fluorescent protein (EGFP) knock-in mice (Sox9-3'EGFP) and Sox9-EGFP/EGFP null chimeras. EGFP-positive cells of Sox9-3'EGFP knock-in and Sox9-EGFP/EGFP null chimeric embryos harvested from limb buds at embryonic day 12.5 were sorted using a FACSAria flow cytometer (Becton-Dickinson). Total RNA of sorted cells was extracted using the RNeasy Mini Kit (QIAGEN) and amplified according to the instructions provided by Affymetrix. Microarray analysis using the Affymetrix Mouse Genome 430 2.0 Array was performed according to the manufacturer's instructions.

2011-02-16 | E-MTAB-537 | biostudies-arrayexpress

Identification of Sox9-Regulated Pathways During Early Pancreas Organogenesis

Project description:Sox9 target genes were identified by comparing gene expression in Sox9-ablated versus wild-type pancreata using microarray analysis. Sox9 was conditionally ablated in the developing pancreas via recombination of a Sox9-flox allele (Kist et al., 2002) using the Pdx1-Cre transgene (Gu et al., 2002). Total RNA was isolated and pooled from dorsal pancreatic epithelia of e12.5 Sox9fl/fl; Pdx1-Cre (mutant) versus Sox9fl/fl (wild-type) littermates for three biological replicates.

2012-08-01 | E-GEOD-28669 | biostudies-arrayexpress

Identification of Sox9-Regulated Pathways During Pancreas Development

Project description:This SuperSeries is composed of the following subset Series: GSE28669: Identification of Sox9-Regulated Pathways During Early Pancreas Organogenesis GSE28670: Identification of Sox9-Regulated Pathways During the Secondary Transition Stage of Pancreas Development Refer to individual Series

2012-08-01 | E-GEOD-28671 | biostudies-arrayexpress

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