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Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (ChIP-Seq)

ABSTRACT: Characterization of the activities of the transcription factors that AP3 and PI encode throughout flower development using perturbation and ChIPSeq assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE38358 | GEO | 2012/06/01

SECONDARY ACCESSION(S): PRJNA167820

REPOSITORIES: GEO

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Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (ChIP-Seq)

Project description:Characterization of the activities of the transcription factors that AP3 and PI encode throughout flower development using perturbation and ChIPSeq assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. Examination of genomic regions bound by fully functional AP3-GFP and PI-GFP proteins at approx floral stage 4-5 as compared to a negative control sample

2012-06-01 | E-GEOD-38358 | biostudies-arrayexpress

Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (mRNA)

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Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (mRNA)

Project description:Characterization of the activities of the transcription factors that AP3 and PI encode throughout flower development using perturbation assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. The series contains two types of perturbation experiments, static permutations (null alleles pi-1 and ap3-3, respectively) and dynamic perturbations (temperature-sensitive ap3-1 allele). Two conditions (i.e. genotypes: ap3-3 or pi-1 homozygous in the FIS vs ap3-3 or pi-1 heterozygous in the FIS, respectively / ap3-1 plants vs AP3 plants shifted from 16degrees to 27 degrees ) at three developmental stages each

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Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA

Project description:This SuperSeries is composed of the following subset Series: GSE38358: Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (ChIP-Seq) GSE38362: Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (mRNA) Refer to individual Series

2012-05-31 | E-GEOD-38363 | biostudies-arrayexpress

Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA.

Project description:How different organs are formed from small sets of undifferentiated precursor cells is a key question in developmental biology. To understand the molecular mechanisms underlying organ specification in plants, we studied the function of the homeotic selector genes APETALA3 (AP3) and PISTILLATA (PI), which control the formation of petals and stamens during Arabidopsis flower development. To this end, we characterized the activities of the transcription factors that AP3 and PI encode throughout flower development by using perturbation assays as well as transcript profiling and genomewide localization studies, in combination with a floral induction system that allows a stage-specific analysis of flower development by genomic technologies. We discovered considerable spatial and temporal differences in the requirement for AP3/PI activity during flower formation and show that they control different sets of genes at distinct phases of flower development. The genomewide identification of target genes revealed that AP3/PI act as bifunctional transcription factors: they activate genes involved in the control of numerous developmental processes required for organogenesis and repress key regulators of carpel formation. Our results imply considerable changes in the composition and topology of the gene network controlled by AP3/PI during the course of flower development. We discuss our results in light of a model for the mechanism underlying sex-determination in seed plants, in which AP3/PI orthologues might act as a switch between the activation of male and the repression of female development.

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Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (ChIP)

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Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (expression)

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Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (expression)

Project description:Characterization of the activities of the transcription factor that AG encodes throughout flower development using perturbation assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. The series contains static perturbation experiments (null allele ag-1), and amiRNA knockdown experiments. Two conditions (i.e. perturbation experiments of ag-1 homozygous in the FIS vs ag-1 heterozygous in the FIS across three developmental stages. amiRNA knockdown experiments induced amiRNA expression vs. uninduced.

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Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (ChIP)

Project description:Characterization of the activities of the transcription factor that AG encodes throughout flower development using perturbation assays and ChIP-Seq in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. Examination of genomic regions bound by fully functional AG-GFP protein at approx floral stage 4-5 as compared to a negative control sample.

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Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-06-01 | GSE38363 | GEO