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Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (expression)

ABSTRACT: Characterization of the activities of the transcription factor that AG encodes throughout flower development using perturbation assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. The series contains static perturbation experiments (null allele ag-1), and amiRNA knockdown experiments.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE44919 | GEO | 2013/07/01

SECONDARY ACCESSION(S): PRJNA196760

REPOSITORIES: GEO

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Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (expression)

Project description:Characterization of the activities of the transcription factor that AG encodes throughout flower development using perturbation assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. The series contains static perturbation experiments (null allele ag-1), and amiRNA knockdown experiments. Two conditions (i.e. perturbation experiments of ag-1 homozygous in the FIS vs ag-1 heterozygous in the FIS across three developmental stages. amiRNA knockdown experiments induced amiRNA expression vs. uninduced.

2013-07-01 | E-GEOD-44919 | biostudies-arrayexpress

Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (ChIP)

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Control of Reproductive Floral Organ Identity Specification in Arabidopsis by the C Function Regulator AGAMOUS (ChIP)

Project description:Characterization of the activities of the transcription factor that AG encodes throughout flower development using perturbation assays and ChIP-Seq in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. Examination of genomic regions bound by fully functional AG-GFP protein at approx floral stage 4-5 as compared to a negative control sample.

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Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (mRNA)

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Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (mRNA)

Project description:Characterization of the activities of the transcription factors that AP3 and PI encode throughout flower development using perturbation assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. The series contains two types of perturbation experiments, static permutations (null alleles pi-1 and ap3-3, respectively) and dynamic perturbations (temperature-sensitive ap3-1 allele). Two conditions (i.e. genotypes: ap3-3 or pi-1 homozygous in the FIS vs ap3-3 or pi-1 heterozygous in the FIS, respectively / ap3-1 plants vs AP3 plants shifted from 16degrees to 27 degrees ) at three developmental stages each

2012-05-31 | E-GEOD-38362 | biostudies-arrayexpress

Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (ChIP-Seq)

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Molecular basis for the specification of floral organs by APETALA3 and PISTILLATA (ChIP-Seq)

Project description:Characterization of the activities of the transcription factors that AP3 and PI encode throughout flower development using perturbation and ChIPSeq assays in combination with a floral induction system (FIS) that allows a stage-specific analysis of flower development. Examination of genomic regions bound by fully functional AP3-GFP and PI-GFP proteins at approx floral stage 4-5 as compared to a negative control sample

2012-06-01 | E-GEOD-38358 | biostudies-arrayexpress

The KNOXI gene SHOOT MERISTEMLESS regulates floral fate in Arabidopsis

Project description:Plants have evolved a unique and conserved developmental program that enables the conversion of leaves into floral organs. Elegant genetic and molecular work has identified key regulators of floral meristem identity. However, further understanding of flower meristem specification has been hampered by redundancy and by pleiotropic effects. The KNOXI gene STM transcription factor is a well-characterized regulator of shoot apical meristem maintenance. stm loss-of-function mutants arrest shortly after germination, and therefore the knowledge on later roles of STM, including flower development, is limited. Here, we uncover a role for STM in the specification of flower meristem identity. Silencing STM in the AP1 expression domain in the ap1-4 mutant background resulted in a complete leafy-like flower phenotype and an intermediate stm-2 allele enhanced the floral meristem identity phenotype of ap1-4. Transcriptional profiling of STM perturbation suggested that STM activity affects multiple meristem identity and flower transition genes, among them the F-Box gene UFO. In agreement, stm-2 enhanced the ufo-2 floral meristem fate phenotype, and ectopic UFO expression rescued the leafy flowers in genetic backgrounds with compromised AP1 and STM activities. This work suggests a molecular mechanism that underlies the activity of STM in the specification of flower meristem identity.

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AGAMOUS-GR time-course experiment

Project description:Inflorescences of ag-1;35S::AG-GR plants were treated for 0,4,8,10, or 16 hours with dexamethasone or were mock-treated. Two biologically independent sets of samples were generated and RNA probes derived from these samples were co-hybridized to a cDNA array enriched for flower-specific transcripts. Keywords: time-course

2004-07-15 | GSE1424 | GEO

Target genes of AGAMOUS during early flower development in Arabidopsis

Project description:Floral organs, whose identity is determined by specific combinations of homeotic genes, originate from a group of undifferentiated cells called the floral meristem. In Arabidopsis, the homeotic gene AGAMOUS (AG) terminates meristem activity and promotes development of stamens and carpels. To understand the program of gene expression activated by AG, we followed genome-wide expression during early stamen and carpel development. Keywords: Developmental time course

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