Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome response to the deletion of Gcr1, Nup84 or Nup84 & Gcr1 (double deletion)

ABSTRACT: This experiment compares the transcriptional response of yeast cells to the deletion of Gcr1, Nup84, or both, to isogenic wild type strains grown in YPD at 23C. The goal was to identify misregulated target genes and analyze the functional classifications of those targets. Keywords: cell type comparison (knock-outs)

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE3853 | GEO | 2007/08/22

SECONDARY ACCESSION(S): PRJNA94079

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptome response to gcr1 deletion in raffinose, galactose, and glucose

Project description:This experiment compares transcriptomic response of yeast cells to the deletion of Gcr1 to isogenic wild type strains, grown in YEP(3%)R at 23C. The goal was to identify misregulated target genes and analyze the functional classifications of those targets. Keywords: cell type comparison

2006-11-27 | GSE5027 | GEO

ChIP-seq for two forms of Gcr1, during exponential phase and diauxic shift

Project description:Gcr1, the most important transcription factor for glycolytic genes in Saccharomyces cerevisiae, was recently revealed to have two isoforms, Gcr1(U) and Gcr1(S), produced from unspliced and spliced transcripts, respectively. We generated strains expressing only Gcr1(U) or Gcr1(S) form using the CRISPR/Cas9 system, and added a TAP tag protein to the Gcr1(U) and Gcr1(S), using HIS3MX6 marker system. We conducted ChIP-seq for both strains during the glycolytic phase or after the diauxic shift.

2020-07-07 | GSE153841 | GEO

RNA-seq for two forms of Gcr1, during exponential phase and diauxic shift

Project description:Gcr1, the most important transcription factor for glycolytic genes in Saccharomyces cerevisiae, was recently revealed to have two isoforms, Gcr1(U) and Gcr1(S), produced from unspliced and spliced transcripts, respectively. We generated strains expressing only Gcr1(U) or Gcr1(S) form using the CRISPR/Cas9 system, and performed RNA-seq for the two strains. Since Gcr1 works as a glycolytic gene transcription factor, we extracted whole RNA at the exponential phase (6 hrs after incubation) and the diauxic shift (12 hrs after incubation).

2020-07-07 | GSE153842 | GEO

Response of gcr1 null mutant to glucose

Project description:This experiment was done to detect the transcriptomic defects in the gcr1 null mutant, relative to an isogenic wild type, in the immediate response to glucose. Keywords: time course Both gcr1 mutant and wild type cells were grown in YEP containing 3% pyruvate to early/mid log phase. The 0min sample was taken just prior to glucose addition. After addition of glucose, samples were taken at 20min and 60min. Total RNA was extracted for both strains and expression was measured by competitive hybridization of the mutant and wild type labeled cRNA for each time point.

2010-06-30 | E-GEOD-5575 | biostudies-arrayexpress

Response of gcr1 null mutant to glucose

2006-11-27 | GSE5575 | GEO

Transcription profiling of yeast Gcn4 and Arr1 deletion mutant as well as Gcn4 Arr1 double deletion mutant.

Project description:Transcription profiling of wild type yeast strain as well as strains carrying a deletion of Gcn4, Arr1 or both. Gene expression in rich medium (YPD) and under osmotic stress conditions (YPD + 0.8M NaCl) was compared.

2012-06-25 | E-MEXP-3566 | biostudies-arrayexpress

Differential gene expression in the grf10Δ mutant of Candida albicans in high copper containing growth medium

Project description:To identify genes in that are misregulated in the grf10Δ mutant of Candida albicans in response to growth in high copper, we compared expression patterns of the grf10-null mutant relative to the isogenic WT in YPD medium supplemented with and without 12 mM CuSO4.

2023-03-20 | GSE223217 | GEO

Transcriptome profiling reveals a genomewide role of G-proteins

Project description:Knock out mutants of GPA1 (gpa1-5), GCR1 (gcr1-5) as well as their double mutant (gpa1-5 gcr1-5) were used to obtain genomewide transcriptome profile to deduce genomewide effect of both the subunits alone or in combination. Heterotrimeric G-protein signaling pathways have been implicated in a variety of biological processes in plants. While an impressive range of important plant responses are known to be mediated by G-protein signaling pathways, the genes that respond through these pathways are not yet known completely. More importantly, the role of G-proteins in mediating plant metabolic regulation, which is well established in animal systems, has not received adequate attention. The availability of loss-of-function G_alpha (GPA1) and G-protein coupled receptor (GCR1) mutant(s) as well as their double mutant in Arabidopsis provided an opportunity for a genome-wide microarray analysis in the current study for the first time. transcriptome profile of mutants gpa1-5, gcr1-5 and double mutant gpa1-5 gcr1-5 were compared to that of the corresponding wild type, ws2. biological duplicates of each sample wa used and hybridized using single color array

2015-02-25 | E-GEOD-40217 | biostudies-arrayexpress

Transcriptional effects of the IWR1 deletion

Project description:Analysis of the genome-wide transcrptional effects caused by the deletion of the IWR1 gene by comparing the transcriptional profile of a iwr1 mutant strain with the isogenic wild type strain when cells were exponentially grown in YPD medium.

2009-10-13 | GSE17303 | GEO

Transcriptome profiling reveals a genomewide role of G-proteins

2015-02-25 | GSE40217 | GEO