Genome wide co-localization of Polycomb orthologs and their effects on gene expression in human fibroblasts
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ABSTRACT: We have identfied antibodies that support chromatin immunoprecipitation (ChIP) of 11 components of Polycomb repressive complex 1 (PRC1) and shown that multiple variants of PRC1 associate with the same DNA. By performing ChIP-seq with antibodies against CBX6, CBX7, CBX8, RING1 and RING2, in two strains of human fibroblasts, we find that all five proteins co-localize at multiple sites in the genome. The binding profiles have inherent architecture that is conserved between the two strains, but there are also a substantial number of differences between the strains, possibly reflecting their anatomical position. The presence of PcG proteins does not equate with gene silencing and their genomic position does not change at senescence.
ORGANISM(S): Homo sapiens
PROVIDER: GSE40740 | GEO | 2013/12/19
SECONDARY ACCESSION(S): PRJNA174794
REPOSITORIES: GEO
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