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Monocyte-like THP-1 Cells: Trichosporon asahii Infected vs Control

ABSTRACT: Transcriptional profiling of Monocyte-like THP-1 Cells with Trichosporon asahii Infected THP-1 Cells comparing untreated THP-1 cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE41983 | GEO | 2012/11/03

SECONDARY ACCESSION(S): PRJNA178724

REPOSITORIES: GEO

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Monocyte-like THP-1 Cells: Trichosporon asahii Infected vs Control

Project description:Transcriptional profiling of Monocyte-like THP-1 Cells with Trichosporon asahii Infected THP-1 Cells comparing untreated THP-1 cells Two-condition experiment,Trichosporon asahii Infected THP-1 Cells vs THP-1 Cells. Biological replicates: 3 infected THP-1 Cells, independently grown and harvested.One replicate per array.

2012-11-03 | E-GEOD-41983 | biostudies-arrayexpress

Human THP-1 Cells: Uninfected vs. Coxiella burnetii NMII infected compared to Uninfected with Chloramphenicol vs. Coxiella burnetii NMII infected with Chloramphenicol

Project description:A comparison was made between the THP-1(Human monocytic leukemia cells - TIB-202; ATCC) transcriptional responses of; (i) uninfected versus Coxiella burnetii NMII infected and (ii) uninfected versus Coxiella burnetii NMII infected THP-1 cells transiently treated with bacteriostatic levels (10μg/ml) of chloramphenicol (CAM). Briefly, infections were initiated and cultured in parallel with uninfected cells. At 48 hours post infection (hpi), media containing CAM (10μg/ml) was added to one set of cells (uninfected and infected THP-1 cells) and culturing was continued. The other set of cells were mock treated with normal media. Total RNA was isolated at 72 hpi from all conditions. Microarrays were performed for both condition sets and the results from each of the two microarrays were compared to define the host genes modulated by de novo C. burnetii NMII protein synthesis.

2010-10-01 | GSE23665 | GEO

Gene expression data from THP-1 cells exposed to exosomes from acetaminophen-treated or vehicle-treated hepatocytes or corresponding mock- or medium-controls

Project description:In the present study we examined the effect of hepatocyte-derived exosomes (HDE) on innate immune cells by treating a human monocyte cell line (THP-1 cells) with exosomes collected from the culture medium of acetaminophen (APAP)-treated and untreated primary human hepatocytes and comparing the cytokine response to THP-1 cells treated with mock- and medium-controls. We profiled the gene expression changes observed in the HDE-exposed monocytes prior to LPS stimulation. Findings from our research suggest that HDE may be essential for maintaining immune homeostasis.

2019-12-31 | GSE125690 | GEO

Murine primary dendritic cells: Control vs. Influenza-infected vs. polyI:C stimulated.

Project description:MIcroRNA expression profiling of primary murine splenic dendritic cells (Flt3L expanded) comparing untreated cells to cells infected with Influenza A or stimulated with polyI:C in vitro.

2012-12-05 | GSE36316 | GEO

Expresson from human THP-1 stimulated with IFN-g and/or infected with TB

Project description:Investigation of interacting effects of TB and IFN-g on gene expression regulation within human macrophage-like THP-1 cells To investigate the effect TB infection has on THP-1 macrophageâ??s response to IFN-g post-infection, in multiple experiments, THP-1 cells were exposed to each of the following four conditions: not treated, IFN-Î³ treated, M. tuberculosis infected and M. tuberculosis infected then IFN-Î³ treated. Total cellular RNA was then extracted. Recovered RNAs were pre-qualified for reproducibility by comparing qRT-PCR results of IFIT-2 and IP10 between replicate experiments. Four most reproducible replicate experiments were then selected for genome-wide mRNA abundance assays using Affymetrix microarray-based technology.

2010-03-09 | E-GEOD-17477 | biostudies-arrayexpress

Human melanocytes: Control vs. RAS or BRAF Infected

Project description:Transcriptional profiling of human melanocytes comparing control untreated cells with cells infected with different oncogenic mutations. Goal was to determine the effects of melanoma-associated mutation overexpression on global melanocyte gene expression.

2016-01-31 | GSE50911 | GEO

Mouse Macrophage Cells: Control vs. Burkholderia pseudomallei infected cells

Project description:Transcriptional profiling of mouse macrophage cells comparing control untreated cells with macrophage cells infected with Burkeholderia pseudomallei. Goal was to determine the effects of bacterial infection on global macrophage gene expression.

2011-07-15 | GSE30642 | GEO

Macrophages THP-1: Control vs Colpvp

Project description:Transcriptional array of macrophages cells comparing control untreated vs colpvp treated for 24hrs

2024-04-01 | GSE262737 | GEO

Differentially regulated genes induced in Mycobacterium avium subspecies paratuberculosis by in vitro infection of THP-1 human macrophage cell line

Project description:Transcriptional profile of Mycobacterium avium subspecies paratuberculosis in in vitro THP-1 (ATCC TIB-202) cell line infection, comparing bacteria grown in host medium and intracellular bacteria recovered from infected THP-1 cells.

2012-06-01 | GSE32242 | GEO

TaqMan Human miRNA array profile of Mycobacterium tuberculosis H37Rv infected THP-1 differentiated macrophages cells.

Project description:Differentially regulated miRNA candidates in H37Rv infected THP-1 cells were analysed with respect to uninfected THP-1 reference samples. THP-1 cells are monocytes differentiated to macrophages after treatment with PMA for 48 hrs. Total RNA was isolated from infected THP-1 cells after 24 hrs of infection, cDNA was synthesized for TLDA real time PCR reaction using TaqMan MicroRNA Reverse Transcription kit and Megaplex Human Pool A and Pool B stem loop RT primers (version 3.0) as per manufacturer’s protocol. Further real time reaction was performed on QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems) by using cDNA (without pre-amplification) on TLDA card A and card B (version 3.0).

2017-04-20 | GSE94007 | GEO

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