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Analyses of lung early responses to RSV in the neonatal environment reveal key roles for DC and type I IFN in controlling immunopathologic imprinting.

ABSTRACT: The gene expression profile of neonatal versus adult lung, 2 days after an RSV or mock infection was analyzed using whole mouse genome agilent microarrays.

ORGANISM(S): Mus musculus

PROVIDER: GSE42759 | GEO | 2015/12/21

SECONDARY ACCESSION(S): PRJNA183068

REPOSITORIES: GEO

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Analyses of lung early responses to RSV in the neonatal environment reveal key roles for DC and type I IFN in controlling immunopathologic imprinting.

Project description:The gene expression profile of neonatal versus adult lung, 2 days after an RSV or mock infection was analyzed using whole mouse genome agilent microarrays. Total lung RNA were extracted, labeled with Cy3 and hybridized on Agilent G4122F slides. We used 4 biological replicates per condition.

2015-12-21 | E-GEOD-42759 | biostudies-arrayexpress

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2012-03-12 | E-GEOD-32138 | biostudies-arrayexpress

The response of human primary airway epithelial cells to Influenza or RSV infection [hAECs_Illumina]

Project description:To investigate how human airway epithelial cells respond to Influenza or RSV infection, we harvested airway epithelial cells from the mainstream bronchi of human donors and cultured them as previously described (Pickles et al,1998) in a polarized system that resembles the in vivo mucociliary pseudostratified epithelium. Quadruplicate hAEC cultures were infected with 2X105 PFUs Influenza A (Udorn) or with 1x106 PFUs RSV for 2h or mock inoculated and harvested 24h after Influenza infection and 48h after RSV infection. Quadruplicate polarized airway epithelial cell cultures were infected with 2x10^5 PFUs of Influenza A (Udorn) for 2h or infected with 1x10^6 PFUs RSV and harvested 24 h post infection for Influenza or 48h post infection for RSV.Duplicate cultures were used as controls for each condition (Two cultures were mock treated mor 2h and harvested after 24h for the Influenza infection and 2 cultures were mock treated for 2h and harvested after 48 hours for the RSV infection.Total RNA was harvested and gene expression was studied using Genespring GX v7.3.1.

2012-03-12 | E-GEOD-32139 | biostudies-arrayexpress

The response of human primary airway epithelial cells to Influenza or RSV infection [hAECs_Illumina]

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RSV vs BSA

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2014-06-21 | E-GEOD-58425 | biostudies-arrayexpress

Transcription profiling of lung and bronchial lymph node from mice infected with Respiratory Syncytial Virus (RSV)

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