Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from adult male and female Drosophila melanogaster antenna, proboscis, and front leg

ABSTRACT: Sex-specific gene expression in sensory organs may play an important role in mating and foraging behavior. We used long-oligonucleotide microarrays to compare gene expression profiles of males and females in three adult appendages that carry large numbers of chemosensory organs – antenna, proboscis, and front leg. Keywords: tissue-specific expression profiles

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE5189 | GEO | 2007/06/22

SECONDARY ACCESSION(S): PRJNA96527

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Expression data from adult male and female Drosophila melanogaster antenna, proboscis, and front leg

Project description:Sex-specific gene expression in sensory organs may play an important role in mating and foraging behavior. We used long-oligonucleotide microarrays to compare gene expression profiles of males and females in three adult appendages that carry large numbers of chemosensory organs â antenna, proboscis, and front leg. Keywords: tissue-specific expression profiles Drosophila isogenic line WI89 was used. Adult male and female antennae (a3+arista), front legs (from distal tibia down), and proboscises were dissected at 1-2 hours after eclosion. Total RNA was extracted and the mRNA fraction was amplified by reverse transcription followed by in vitro transcription with T7 RNA polymerase.

2010-06-10 | E-GEOD-5189 | biostudies-arrayexpress

Comparative transcriptomics of chemosensory tissues between Drosophila species

Project description:These data contain RNA-seq samples generated from the main chemosensory organs of closely related Drosophila species (D. melanogaster, D. sechellia, D. simulans, D. santomea, D. erecta, and D. suzukii) from larava (head) and adults (antenna, forelegs, proboscis with maxillary palps, ovipositors (female only) for both males and females. The purpose for generating these data was to carry out evolutionary analyses of gene expression differences between the six species.

2023-04-13 | E-MTAB-12656 | biostudies-arrayexpress

RNA-seq of adult Heliconius melpomene rosina male and female chemosensory tissues: antennae, labial palps and proboscis, and legs

Project description:The purpose of this experiment was to compare RNA-seq profiles of adult male and female butterfly chemosensory tissues to identify tissue- and sex-specific differences in gustatory and olfactory gene expression. Three biological replicates per sex were produced from individual Heliconius melpomene rosina butterflies. For the antennal libraries, both antennae were used, for the labial palps + proboscis libraries both labial palps and each proboscis was used, and for the leg libraries all six legs were used.

2014-02-14 | E-MTAB-1500 | biostudies-arrayexpress

Shotgun proteomics of chemosensory organs of Varroa destructor mite

Project description:Aims of the project is the identification of soluble chemosensory proteins in the olfactory organs of the honeybee mite Varroa destructor. Different families of soluble proteins acting as carriers for odorants have been identified in hexapods, while very limited information is available for other terrestrial Arthropoda. In mites and ticks olfactory organs are located on the distal part of the forelegs and on the capitulum appendages. These two body tagmata and the second pair of legs as control, have been dissected out of phoretics and reproductives Varroa destructor females and protein extracts have been analysed through shotgun proteomics. Protein identification and relative quantification (Label-free quantification, LFQ) has been performed using MaxQuant (version 1.5.8.3). Among the proteins more abundant in appendages bearing chemosensory organs, considering as a model hexapoda soluble olfactory proteins, we have searched for small secreted proteins whose structure include a hydrophobic binding pocket.

2018-04-12 | PXD008679 | Pride

Manduca sexta differential gene expression, male & female antenna, larval midgut

Project description:To allow estimation of the complexity of the antennal transcriptome between sexes as well as in comparison with a non-antennal tissue, microarrays were designed based on an assembly of new 454 sequence data from the antenna and publicly available data including 454 data from the larval midgut. The microarrays were hybridized with samples generated from the repsecitve tissues from 3 (antenna) and 5 (midgut) animals per sample, with four independent samples per sex (antenna) and tissue (midgut).

2011-03-01 | GSE27470 | GEO

Crustacean leg regeneration restores cell diversity

Project description:Animals can regenerate complex organs, yet this frequently results in imprecise replicas of the original structure. In the crustacean Parhyale, we examine the fidelity of leg regeneration using cellular composition and cell molecular profiles. Single-nuclei sequencing shows that regenerated and uninjured legs are indistinguishable in terms of cell type composition and transcriptional profiles.

2022-06-30 | GSE206980 | GEO

Drosophila melanogaster

Project description:Expression data from adult male and female Drosophila melanogaster antenna, proboscis, and front leg

| PRJNA96527 | ENA

Identification of Genes Displaying Sex- and Tissue-biased Expression Patterns in the Monarch Butterfly (Danaus plexippus) with an Emphasis on Chemosensory Perception

Project description:Monarch butterflies (Danaus plexippus) rely on milkweeds as larval host plants. Host plant seeking and verification by female butterflies may be mediated by gustatory (GRs) and olfactory receptors (ORs). Here we employed RNA-Seq, bioinformatics and RT-qPCR techniques to identify sex- and tissue-specific gene expression. We focused on chemosensation related genes and pathways, including putative ORs, GRs, ionotropic receptors (IRs), odorant-binding proteins, chemosensory proteins, and steroid hormone mediated signaling in specific chemosensory tissues (i.e., antennae, legs and proboscis). Twelve butterflies evenly split between males and females were caught and used for RNA extraction. Tissue-specific sequencing libraries were prepared and sequenced using Illumina NovaSeq 6000 or HiSeq 3000, generating 2 billion 150-bp, paired-end reads. Many more genes and gene sets were differentially expressed between tissue types than between sexes. A total of 148 chemosensation-related genes exhibited sex- and/or tissue-biased expression. RT-qPCR of a small set of genes confirmed their differential expression between tissue types or between males and females. These findings laid a solid foundation for further investigations into the biological roles of these identified genes underlying chemosensation-mediated behaviors such as foraging and reproduction.

2021-06-01 | GSE150359 | GEO

Transcriptomic analysis of Drosophila leg disc associated myoblasts

Project description:Adult leg muscle precursors are associated to leg imaginal disc, here we provide bulk RNA sequecing of FACS sorted myoblasts from dissected imaginal leg discs at beginning of pupation.

2024-06-28 | GSE245192 | GEO

A common set of DNA regulatory elements shapes Drosophila appendages

Project description:We mapped open chromatin by FAIRE-seq and measured gene expression by RNA-seq in 3 types of Drosophila samples: staged whole embryos, imaginal discs, pharate appendages. We first demonstrate that regions of open chromatin precisely define regions of enhancer activity in developing embryos. In contrast to the dynamic changes in open chromatin observed between different stages of embryogenesis, we found that the open chromatin profiles in wing, leg, and haltere imaginal discs are nearly identical. This was also true again later in development, where the adult appendages also share nearly identical open chromatin profiles. Therefore, at a given developmental time point, different appendages are specified using a shared set of DNA regulatory elements. However, from one time point to the next, the set of accessible regulatory elements changes. Open chromatin profiles in appendage imaginal discs are almost entirely different than those of the adult appendages. We propose that master regulator transcription factors create morphologically distinct structures by differentially influencing the function of the same set of DNA regulatory modules. Open chromatin profiling during Drosophila development: 3 stages of embryogenesis (2-replicates each); wing, leg, and haltere 3rd instar imaginal discs (3-replicates each); 3rd larval central nervous system (2-replicates); eye-antennal imaginal discs (2-replicates); wing, leg, and haltere pharate appendages (2-replicates each); Genomic DNA Inputs. Sequencing performed on Illumina GAII and HiSeq.

2013-11-07 | E-GEOD-38727 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data