Expression data from OT-I CD8 T cells activated with DC in the presence or absence of CpG-Induced Inflammation
Ontology highlight
ABSTRACT: Inflammatory cytokines promote the accumulation of activated CD8 T cells. Here, we transfer 600 OT-I CD8 T cells iv into naïve C57BL/6 hosts. One day later, 500,000 LPS-matured and OVA257 peptide-coated DC were injected iv into OT-I CD8 T cell seeded hosts with (DC+CpG) or without (DC). Other seeded mice were infected with 2x10^4 virulent Listeria monocytogenes (vLM-OVA) iv. OT-I CD8 T cells were harvested from the spleen, flow sort purified, then RNA was extracted using RNeasy (Qiagen) kit. Naive OT-I CD8 T cells (Naive) were purified from the spleens of OT-I transgenic mice. Each group had three independent biological replicates.Transcriptomes were compared using DAVID analysis (with genes scoring FDR<0.01) and GSEA analysis.
ORGANISM(S): Mus musculus
PROVIDER: GSE53200 | GEO | 2013/12/10
SECONDARY ACCESSION(S): PRJNA231091
REPOSITORIES: GEO
ACCESS DATA