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Comparison of the expression profiles of 630E strain and an Hfq antisense after 7.5h of growth in Tyt

ABSTRACT: Transcriptional profiling of C. difficile 630E strain vs. an Hfq antisense after 7.5h of growth inTYt.

ORGANISM(S): Clostridioides difficile

PROVIDER: GSE55273 | GEO | 2014/07/15

SECONDARY ACCESSION(S): PRJNA239038

REPOSITORIES: GEO

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Comparison of the expression profiles of 630E strain and an Hfq antisense after 7.5h of growth in Tyt

Project description:Transcriptional profiling of C. difficile 630E strain vs. an Hfq antisense after 7.5h of growth inTYt. Two-conditions experiments, 630E strain vs. Hfq antisense grown in TYt, 4 biological replicates for each condition.

2014-07-15 | E-GEOD-55273 | biostudies-arrayexpress

Comparison of the expression profiles of 630E strain and a hfQ mutant after 7.5h of growth in Tyt

Project description:Clostridium difficile is an emergent human pathogen and the most common cause of nosocomial diarrhea. Our recent deep-sequencing data strongly suggest the importance of RNA-based mechanisms for the control of gene expression in C. difficile. The RNA chaperon protein Hfq is a key component of post-transcriptional regulatory network in various bacterial systems. In an effort to understand the function of Hfq in C. difficile, we have constructed and characterized an Hfq-depleted strain in this bacterium. We present evidence that Hfq is necessary for the normal cell growth, for the establishment of bacillary form and might be involved in metabolic adaptations, stress response and sporulation, important during C. difficile infection cycle. In accordance to these phenotypic changes, our transcriptome analysis revealed pleiotropic effects of Hfq on gene expression in C. difficile. This microarray analysis showed altered expression of genes encoding cell wall and membrane transport components, sporulation proteins, as well as stress response, transcriptional regulator genes and genes of unknown function. Remarkably, a great number of genes belonging to the regulon dependent on sporulation-specific sigma K factor were generally up-regulated in the strain depleted for Hfq. Many important metabolic pathways were also affected by Hfq depletion. Finally, the altered accumulation of several previously identified sRNAs suggests potential involvement of Hfq in these regulatory RNA function. Altogether, these results provide evidence for the pleiotropic role of Hfq protein in C. difficile physiology expanding our knowledge of Hfq-dependent regulation in Gram-positive bacteria. Two-conditions experiments, 630E strain vs. hfQ mutant grown in TYt, 4 biological replicates for each condition.

2014-07-15 | E-GEOD-49168 | biostudies-arrayexpress

Comparison of the expression profiles of 630E strain and a hfQ mutant after 7.5h of growth in Tyt

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Comparison of the expression profiles of 630E strain after 4h and 10h of growth

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Comparison of the expression profiles of 630E strain and a sigD mutant after 6h of growth.

Project description:Transcriptionnal profiling of C. difficile 630E strain vs a sigD mutant after 6h of growth in BHI

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Comparison of the expression profiles of 630E strain and a sigH mutant after 10h of growth

Project description:Transcriptionnal profiling of C. difficile 630E strain vs a sigH mutant after 10h of growth in TY

2011-08-22 | GSE25475 | GEO

Comparison of the expression profiles of 630E strain and a sig54 mutant after 6h of growth

Project description:Transcriptional profiling of C. difficile 630E strain vs. a sig54 mutant after 6h of growth in TY.

2020-08-28 | GSE149245 | GEO

Comparison of the expression profiles of 630E strain after 4h and 10h of growth

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2011-08-21 | E-GEOD-25474 | biostudies-arrayexpress

Comparison of the expression profiles of 630E JIR8094 strain and a ccpA mutant after 10h of growth in TY.

Project description:Transcriptionnal profiling of C. difficile 630E JIR8094 strain vs a ccpA mutant after 10h of growth in TY

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Comparison of the expression profiles of 630E strain and a sigH mutant after 10h of growth

Project description:Transcriptionnal profiling of C. difficile 630E strain vs a sigH mutant after 10h of growth in TY Two-conditions experiments, 630E strain 10h vs sigH mutant 10h, 4 biological replicates for each condition

2011-08-21 | E-GEOD-25475 | biostudies-arrayexpress

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