Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Comparison of gene expression by co-cultured WC1+ gammadelta and CD4+ alphabeta T cells

ABSTRACT: Vaccination of animals with leptospiral vaccine results in the generation of antigen-responsive CD4 alphabeta T cells and WC1 gammadelta T cells which proliferate and produce effector cytokines following secondary encounter with antigen. In these studies we compared the gene expression of these antigen-stimulated T cell subsets to each other by microarray. In addition, each activated T cell population was compared to a reference sample, consisting of unstimulated peripheral blood mononuclear cells. Keywords: cell type comparison

ORGANISM(S): Bos taurus

PROVIDER: GSE5603 | GEO | 2006/08/26

SECONDARY ACCESSION(S): PRJNA96889

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Shh is a determinant of gammadelta T-cell development in the thymus

Project description:We compared gammadelta T-cell development and subsets in the thymus of mice in which Shh had been conditionally deleted from thymic epithelial cells using FoxN1-Cre to WT mice, and to transgenic mice in which a constitutively activator (Gli2DN2) or constitutuvely repressor (Gli2DC2) form of Gli2 were expressed under the control of the lck-promotor. We sorted CD27+CD3+TCR-gammadelta+ cells from 4 week old mice and comapred gene expression by RNAsequencing. All mice were on a C57BL/6 background.

2021-04-20 | GSE113468 | GEO

Single cell sequencing of mouse gammadelta T cells

Project description:Mouse gammadelta T cells were sorted from adult C57Bl/6 mouse spleen and lymph nodes and cells were subjected to scRNA-seq

2023-04-01 | GSE228417 | GEO

Microarray analysis of total naïve and YFV-17D specific CD8 T cells in humans

Project description:CD8 T cells play an importart role in adaptive immunity to intracellular pathogens. Naïve CD8 T cells , that have not encountered antigen previously can be identified by virtue of their distinct phenotype. Upon antigenic encounter, they proliferate rapidly and undergo massive reprograming to differentiate to cytotoxic T lymphocytes. The yellow fever live virus vaccine (YF-17D) provides a model primary acute viral infection that can be used to follow this response.Here we characterize the resting, non-activated naive CD8 T cells in nine healthy adults and YF-specific CD8 T cells elicited in response to YF-17D vaccination from the same donors during the effector (2 weeks after vaccination) and memory (5-8 months later) stages.

2017-12-13 | GSE26347 | GEO

Loss and dysfunction of Vdelta2+ gammadelta-low T cells is associated with clinical tolerance to malaria

Project description:Gammadelta T cells from Ugandan children were tested for their responsiveness to stimulation with malaria parasites.

2014-08-26 | GSE55843 | GEO

Transcription profiling of human fetal gammadelta T cells from that cytomegalovirus (CMV) positive or negative

Project description:Response of fetal gammadelta T cells towards infection with human cytomegalovirus (CMV)

2009-12-12 | E-MEXP-2055 | biostudies-arrayexpress

Loss and dysfunction of Vdelta2+ gammadelta-low T cells is associated with clinical tolerance to malaria

Project description:Gammadelta T cells from Ugandan children were tested for their responsiveness to stimulation with malaria parasites. Independent PBMC samples from children were incubated with Plasmodium falciparum-infected red blood cells for 6 h post-treatment, and Vdelta2+ gammadelta T cells were double sorted by FACS directly into an RNA lysis buffer. RNA was isolated, amplified using 2 rounds of linear amplification, and hybridized to Agilent Human 8x60k microarrays as a single color experiment.

2014-08-26 | E-GEOD-55843 | biostudies-arrayexpress

Expression data comparing PLZF+/+, PLZF +/lu, PLZF lu/lu gammadelta NKT cells

Project description:Natural killer (NKT) T cells exhibit tissue distribution, surface phenotype, and functional responses that are strikingly different from those of conventional T cells. The transcription factor PLZF is responsible for most of these properties, as its ectopic expression in conventional T cells is sufficient to confer to them an NKT-like phenotype. The molecular program downstream of PLZF, however, is largely unexplored. Here we report that PLZF regulates the expression of a surprisingly small set of genes, many with known immune functions. This includes several established components of the NKT cell developmental program. Transcriptional program downstream of PLZF in gammadelta NKT cells was analyzed by comparing wt, heterozygous and PLZF-deficient gammadelta NKT cells

2012-11-09 | E-GEOD-42168 | biostudies-arrayexpress

Regulation of global gene expression in human Loa loa infection is a function of chronicity

Project description:Differential gene expression to parasite and nonparasite antigen was seen in infected patients with lifelong exposure to the human filarial parasite Loa loa (endemics) compared with patients who became infected due to temporary residence or travel in an endemic country (expatriates). CD4+ and CD8+ cells were isolated; comparison was done between unstimulated cells from 2 patient groups and between parasite microfilarial (MfAg) and nonparasite streptolysin O (SLO) antigen-stimulated cells with unstimulated cells; Technical replicates (2 unstimulated; 2 each antigen)

2011-11-24 | E-GEOD-31894 | biostudies-arrayexpress

Homo sapiens PBMCs: pre-vaccination (stimulation vs. no stimulation with EV71 antigen) vs. post-vaccination (stimulation vs. no stimulation with EV71 antigen)

Project description:To characterize the primary and recall responses to EV71 vaccines, PBMC from 19 recipients before and after vaccination with EV71 vaccine are collected and their gene expression signatures after stimulation with EV71 antigen were compared. Four-condition experiment,pre-vaccination PBMCs (stimulation vs. no stimulation with EV71 antigen) vs. post-vaccination PBMCs (stimulation vs. no stimulation with EV71 antigen)

2015-09-03 | E-GEOD-72618 | biostudies-arrayexpress

Renal transcriptome profiles from mice infected with Leptospira spp.

Project description:Leptospirosis caused by pathogenic Leptospira spp. leads to kidney damage that may progress to chronic kidney disease. However, how leptospiral infections lead to renal damage is unclear. Here, we apply microarray platforms to determine the murine renal transcriptome-wide investigation of gene expression changes and biological pathways associated with leptospiral infection-related kidney damage. The aims of this study are to investigate a global analysis of renal gene expression associated with renal damage that was induced by leptospiral infection using experimental murine models. The result of microarray analysis showed that 467 and 927 genes to be differentially expressed in mice kidney with pathogenic leptospiral infection after day 7 and 28, respectively. Moreover, the result also showed that the 508 significantly differentially expressed genes in the kidneys of mice after infection with non-pathogenic leptospires at 7 days post-infection, and the 1,067 transcripts significantly differentially expressed in these kidneys at 28 days post-infection. Biological pathways performed using KEGG pathway enrichment analysis (P-value < 1E-5) showed that a total of 6 and 7 pathways were significantly enriched at 7 and 28 days post-infection with pathogenic leptospires, respectively. A total of 25 pathways were significantly enriched at day 28 following the non-pathogenic leptospiral infection. However, none of pathways were found in microarray data at day 7 post-infection with non-pathogenic leptospires. In addition, the antigen processing and presentation pathways was significantly enriched at 7 and 28 days post-infection with pathogenic leptospires, and at day 28 following the non-pathogenic leptospiral infection.

2021-02-23 | GSE111249 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data