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Effect of loss of PKC theta and p50+cRel on gene expression post T-cell stimulation

ABSTRACT: OT-1 Transgenic CD8 T-cells were isolated from spleens of WT, PKCθ theta KO, and p50 cRel DKO mice. The T-cells were either cultured with non-pulsed DC (WT only and signified as "WT - UN") or with BMDCs pulsed with the OVA peptide SIINFEKL (N4) (WT, PKCθ theta KO, and p50 cRel DKO and signified as 'genotype - N4') at a ratio of 1:10 (DC:T-cell) for 18 hours. DCs then were depleted from the culture and RNA was made from the T-cells to measure gene expression at the early / late stage of T-cell activation

ORGANISM(S): Mus musculus

PROVIDER: GSE61758 | GEO | 2014/12/01

SECONDARY ACCESSION(S): PRJNA262048

REPOSITORIES: GEO

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