Effect of Clock deletion in parvalbumin-positive interneurons (PV-cells)
Ontology highlight
ABSTRACT:
ORGANISM(S): Mus musculus
PROVIDER: GSE62956 | GEO | 2015/03/19
SECONDARY ACCESSION(S): PRJNA266288
REPOSITORIES: GEO
Similar Datasets
Project description:This experiment was designed to compare the transcriptomic differences between two parvalbumin (PV) interneuron population of the mouse brain. These two populations have the same embryological origin and share several neurochemical and electrophysiological properties, but differ in their ability to express the glial cell line-derived neurotrophic factor GDNF (negative in cortex and positive in striatum). Two different reporters for PV expressing cells were used: i) a constitutive tdTomato gene inserted in the Pvalb locus, and ii) a PV-Cre; tdTomato model in which fluorescent cells are PV cells expressing Cre recombinase. The comparative gene expression analysis between PV neurons captured from striatum and cortex allowed unraveling differential molecular characteristics of GDNF-synthesizing striatal PV interneurons and their potential role in endogenous GDNF modulation. The specific expression of several genes of interest in the striatal PV interneurons has been validated by other methods (real-time RT-PCR, in situ hibridization, immunohistochemistry).
2020-07-16 | GSE100300 | GEO
Project description:The emerging role of epigenetic mechanisms like DNA methylation executed by DNA methyltransferases (DNMTs) in synaptic function irrevocably raises the question for the targeted subcellular processes and mechanisms. We here sequenced FAC-sorted PVergic interneurons of 6 month and 18month old PV-Cre/tdTomato/Dnmt1loxp wildtype and knockout mice and performed RNA-Seq and MeDIP-Seq.
2020-07-06 | GSE145026 | GEO
Project description:Since many years, we are interested in the regulation of the intraneuronal chloride concentration. We were the first group reporting a full knockout of the KCl-co-transporter KCC2, which dies immediately after birth due to respiratory failure. Notably, KCC2 loss-of-function mutations are associated with inherited febrile seizures, severe genetic generalized epilepsy and epilepsy of infancy with migrating focal seizures. Here, we used our floxed line to study the consequences of the disruption of KCC2 within parvalbumin-positive interneurons in mice. Remarkably, this leads to the disinhibition of PV-positive interneurons as evidenced by a decrease of E-S-Coupling and an increase in the sIPSC frequency. Nevertheless, these mice develop fatal epilepsy with progressive loss of parvalbumin-positive interneurons thus increasing network excitability.
2022-11-02 | E-MTAB-11147 | biostudies-arrayexpress
Project description:The emerging role of epigenetic mechanisms like DNA methylation executed by DNA methyltransferases (DNMTs) in synaptic function irrevocably raises the question for the targeted subcellular processes and mechanisms. We here sequenced FAC-sorted PVergic interneurons of 6 month and 18month old PV-Cre/tdTomato/Dnmt1loxp wildtype and knockout mice and performed RNA-Seq.
2020-07-06 | GSE145025 | GEO
Project description:The emerging role of epigenetic mechanisms like DNA methylation executed by DNA methyltransferases (DNMTs) in synaptic function irrevocably raises the question for the targeted subcellular processes and mechanisms. We here sequenced FAC-sorted PVergic interneurons of 6 month and 18month old PV-Cre/tdTomato/Dnmt1loxp wildtype and knockout mice and performed MeDIP-Seq.
2020-07-06 | GSE145024 | GEO
Project description:Purpose: To identify genes differentially expressed in parvalbumin-positive (PV) interneurons of the hippocampus in non-defetaed (CTRL), stress-susceptible and stress-resilient mice. Method: Translating Ribosome Affinity Purification (TRAP) to isolate RNA from PV+ cells, cDNA synthesis and next generation RNAseq using Illumina Nextseq 500 sequencer. Results: RNA-seq revealed 458 DEGs between non-defeated and resilient mice, 1976 DEGs in non-defeated and susceptible mice, and 3475 DEGs in resilient and susceptible mice.
2021-09-14 | GSE184027 | GEO
Project description:People with schizophrenia show hyperactivity in the ventral hippocampus (vHipp) and we have previously demonstrated distinct behavioral roles for vHipp cell populations. Here, we test the hypothesis that parvalbumin (PV) and somatostatin (SST) interneurons differentially innervate and regulate hippocampal pyramidal neurons based on their projection target. First, we use eGRASP to show that PV-positive interneurons form a similar number of synaptic connections with pyramidal cells regardless of their projection target while SST-positive interneurons preferentially target nucleus accumbens (NAc) projections. To determine if these anatomical differences result in functional changes, we used in vivo opto-electrophysiology to show that SST cells also preferentially regulate the activity of NAc-projecting cells. These results suggest vHipp interneurons differentially regulate that vHipp neurons that project to the medial prefrontal cortex (mPFC) and NAc. Characterization of these cell populations may provide potential molecular targets for the treatment schizophrenia and other psychiatric disorders associated with vHipp dysfunction.
2023-11-09 | GSE244159 | GEO
Project description:Our group has reported that the histone methyltransferase DOT1L is necessary for proper cortical plate development and layer distribution of glutamatergic neurons, however, its specific role on cortical interneuron development has not yet been explored. Here, we demonstrate that DOT1L affects interneuron development in a cell-autonomous manner. Deletion of Dot1l in MGE-derived interneuron precursor cells results in an overall reduction and altered distribution of GABAergic interneurons in the cortical plate at postnatal day (P) 0. Furthermore, we observed an altered proportion of GABAergic interneurons in the cortex and striatum at P21 with a significant decrease in Parvalbumin (PVALB)-expressing interneurons. Altogether, our results indicate that reduced numbers of cortical interneurons upon DOT1L deletion results from altered postmitotic differentiation/maturation.
2023-08-01 | GSE221994 | GEO
Project description:The proliferative niches in the subpallium generate a rich cellular variety fated for diverse telencephalic regions. The embryonic preoptic area (POA) represents one of these domains giving rise to the pool of cortical GABAergic interneurons and glial cells, in addition to striatal and residual POA cells. The migration from sites of origin within the subpallium to the distant targets like the cerebral cortex, accomplished by the adoption and maintenance of a particular migratory morphology, is a critical step during interneuron development, which seems to be regulated partially via DNA methylation-dependent gene expression. To identify genes that are altered by DNA methylation mediated by DNMT1 in POA-derived Hmx3-positive interneurons, we used an Hmx3-Cre/tdTomato/Dnmt1loxP mouse model and FAC-sorted the basal telencephalon at E16. RNA and MeDIP sequencing were performed. MeDIP data are assigned here.
2021-03-09 | GSE146967 | GEO
Project description:The proliferative niches in the subpallium generate a rich cellular variety fated for diverse telencephalic regions. The embryonic preoptic area (POA) represents one of these domains giving rise to the pool of cortical GABAergic interneurons and glial cells, in addition to striatal and residual POA cells. The migration from sites of origin within the subpallium to the distant targets like the cerebral cortex, accomplished by the adoption and maintenance of a particular migratory morphology, is a critical step during interneuron development, which seems to be regulated partially via DNA methylation-dependent gene expression. To identify genes that are altered by DNA methylation mediated by DNMT1 in POA-derived Hmx3-positive interneurons, we used an Hmx3-Cre/tdTomato/Dnmt1loxP mouse model and FAC-sorted the basal telencephalon at E16. RNA and MeDIP sequencing were performed. RNA data are assigned here.
2021-03-09 | GSE146966 | GEO