Genomics

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Roles and Programming of Arabidopsis ARGONAUTE Proteins During Turnip Mosaic Virus Infection


ABSTRACT: AGO protein immunoprecipitation was combined with high-throughput sequencing of associated small RNAs. AGO2, AGO10, and to a lesser extent AGO1 were shown to associate with siRNAs derived from silencing suppressor (HC-Pro)-deficient TuMV-AS9, but not with siRNAs derived from wild-type TuMV. Co-immunoprecipitation and small RNA sequencing revealed that viral siRNAs broadly associated with wild-type HC-Pro during TuMV infection. These results support the hypothesis that suppression of antiviral silencing during TuMV infection, at least in part, occurs through sequestration of virus-derived siRNAs away from antiviral AGO proteins by HC-Pro.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE64911 | GEO | 2015/04/06

SECONDARY ACCESSION(S): PRJNA272491

REPOSITORIES: GEO

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