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Tissue- and stage-specific cellular context regulates Wnt target gene expression subsequent to β-catenin recruitment

ABSTRACT: The aim of our study is to identify direct target genes of Wnt/β-catenin signaling operating in gastrula-stage X. tropicalis embryos. We characterized the genomic loci bound by the Wnt signaling downstream component β-catenin in intact X. tropicalis gastrulae using ChIP-seq. We also determined the normal mRNA expression profile in control (uninjected and control-MO-injected) embryos and altered mRNA expression profiles in two experimental samples: embryos where endogenous wnt8a function was knocked down with wnt8a MO, and where we experimentally re-instated Wnt8a expression with a DNA construct (pCSKA-wnt8a) in the same wnt8a knock down.

ORGANISM(S): Xenopus tropicalis

PROVIDER: GSE72657 | GEO | 2016/04/25

SECONDARY ACCESSION(S): PRJNA294599

REPOSITORIES: GEO

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