Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

E2a is necessary for Smad2/3 dependent transcription and the direct repression of lefty


ABSTRACT: We characterized the binding of Smad2/3 using ChIP-SEQ in both control gastrula-stage X. tropicalis embryos and embryos depleted of the transcription factor E2a (E2a-MO). We also characterized gene expression in control and E2a-depleted embryos by RNA-Seq. For ChIP-Seq, three biological replicates of E2a-MO were performed, and two biological replicates of Control embryos were performed. For RNA-Seq, two biological replicates of E2a-MO were performed, and two biological replicates of Control embryos were performed, and the mean expression levels of each gene were compared. For ChIP-Seq, three biological replicates were performed for E2a-depleted X. tropicalis embryos, and two biological replicates were performed for control gastrula-stage embryos. For RNA-Seq, two biological replicates were performed for both E2a-depleted embryos and control embryos, and the mean expression levels were compared for each gene.

ORGANISM(S): Xenopus (Silurana) tropicalis

SUBMITTER: Andrea Wills 

PROVIDER: E-GEOD-56169 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2014-08-01 | GSE56169 | GEO
2020-01-09 | PXD012770 | Pride
2011-08-23 | E-GEOD-30146 | biostudies-arrayexpress
2014-09-17 | E-GEOD-56000 | biostudies-arrayexpress
2020-12-11 | E-MTAB-9034 | biostudies-arrayexpress
2013-12-05 | E-GEOD-47001 | biostudies-arrayexpress
2022-12-17 | E-MTAB-11978 | biostudies-arrayexpress
2011-12-17 | E-GEOD-34508 | biostudies-arrayexpress
2009-02-09 | E-GEOD-13882 | biostudies-arrayexpress
2022-12-17 | E-MTAB-9158 | biostudies-arrayexpress