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E2a is necessary for Smad2/3 dependent transcription and the direct repression of lefty

ABSTRACT: We characterized the binding of Smad2/3 using ChIP-SEQ in both control gastrula-stage X. tropicalis embryos and embryos depleted of the transcription factor E2a (E2a-MO). We also characterized gene expression in control and E2a-depleted embryos by RNA-Seq. For ChIP-Seq, three biological replicates of E2a-MO were performed, and two biological replicates of Control embryos were performed. For RNA-Seq, two biological replicates of E2a-MO were performed, and two biological replicates of Control embryos were performed, and the mean expression levels of each gene were compared.

ORGANISM(S): Xenopus tropicalis

PROVIDER: GSE56169 | GEO | 2014/08/01

SECONDARY ACCESSION(S): PRJNA242626

REPOSITORIES: GEO

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E2a is necessary for Smad2/3 dependent transcription and the direct repression of lefty

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