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Real-time quantitative PCR analysis of S100A8 and S100A9 effects on lung

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE74129 | GEO | 2016/08/01

SECONDARY ACCESSION(S): PRJNA299118

REPOSITORIES: GEO

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Real-time quantitative PCR analysis of S100A8 and S100A9 effects on lung

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-08-01 | E-GEOD-74129 | biostudies-arrayexpress

Real-time quantitative PCR analysis of S100A8 and S100A9 effects on lung

Project description:Real-time quantitative PCR analysis of S100A8 and S100A9 effects on lung

| PRJNA299118 | ENA

Real-time quantitative PCR analysis of mice lung tissue

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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Real-time quantitative PCR analysis of mice lung tissue [S100A8 time course]

Project description:Recombinant-murine S100A8 were used at 10 mg/50 ml in Hanks Balanced Salt solution (HBSS) or control HBSS administered onto the nares of BALB/C mice. To assess direct effects, mice were sacrificed 1, 4, 6, or 12 h post-inhalation of S100A8. Because S100A8 is reported to initiate proinflammatory responses by ligating TLR4 and/or RAGE, a quantitative PCR array was developed to analyze 49 genes, selected to reflect potential acute inflammatory changes induced by ligation of these receptors. Expression of inflammatory genes was evaluated with the RT-qPCR array. Relative quantities of mRNA in duplicate samples were obtained using the LightCyclerÂ® 480 Software 1.5 and the Efficiency-Method.

2016-08-01 | E-GEOD-74122 | biostudies-arrayexpress

Real-time quantitative PCR analysis of mice lung tissue [S100A9 time course]

Project description:Recombinant-murine S100A9 were used at 10 mg/50 ml in Hanks Balanced Salt solution (HBSS) or control HBSS administered onto the nares of BALB/C mice. To assess direct effects, mice were sacrificed 1, 4, 6, or 12 h post-inhalation of S100A9. Because S100A9 is reported to initiate proinflammatory responses by ligating TLR4 and/or RAGE, a quantitative PCR array was developed to analyze 49 genes, selected to reflect potential acute inflammatory changes induced by ligation of these receptors. Expression of inflammatory genes was evaluated with the RT-qPCR array. Relative quantities of mRNA in duplicate samples were obtained using the LightCyclerÂ® 480 Software 1.5 and the Efficiency-Method.

2016-08-01 | E-GEOD-74128 | biostudies-arrayexpress

Real-time quantitative PCR analysis of mice lung tissue after inhalation of S100A8, S100A8 dimer, S100A9 or S100A8/A9 complex in acute lung [S100A8S100A9 LPS induced ALI]

Project description:LPS (10 Î¼g/50 Î¼l PBS; E. coli Serotype 055:B5, Sigma-Aldrich) was administered onto the nares. Baseline levels of genes in mice treated with vehicle (Hanks Balanced Salt solution (HBSS) and PBS), Intranasal S100s (10 Î¼g/50 Î¼l HBSS) were given 2 h before LPS; control mice received equal volumes of PBS and HBSS. Mice were sacrificed 4 h post LPS inhalation. Expression of inflammatory genes was evaluated with the RT-qPCR array. Relative quantities of mRNA in duplicate samples were obtained using the LightCyclerÂ® 480 Software 1.5 and the Efficiency-Method.

2016-08-01 | E-GEOD-74127 | biostudies-arrayexpress

Real-time quantitative PCR analysis of mice lung tissue [S100A8 time course]

2016-08-01 | GSE74122 | GEO

Real-time quantitative PCR analysis of mice lung tissue [S100A9 time course]

2016-08-01 | GSE74128 | GEO

Real-time quantitative PCR analysis of mice lung tissue

Project description:This SuperSeries is composed of the SubSeries listed below.

2013-11-26 | GSE52720 | GEO

Real-time quantitative PCR analysis of mice lung tissue after inhalation of S100A8, S100A8 dimer, S100A9 or S100A8/A9 complex in acute lung [S100A8S100A9 LPS induced ALI]

Project description:LPS (10 μg/50 μl PBS; E. coli Serotype 055:B5, Sigma-Aldrich) was administered onto the nares. Baseline levels of genes in mice treated with vehicle (Hanks Balanced Salt solution (HBSS) and PBS), Intranasal S100s (10 μg/50 μl HBSS) were given 2 h before LPS; control mice received equal volumes of PBS and HBSS. Mice were sacrificed 4 h post LPS inhalation.

2016-08-01 | GSE74127 | GEO

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