Transcriptomics

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Next-generation sequencing for the fat mass and obesity-associated protein (FTO) binding mRNA


ABSTRACT: Purpose: The goals of this study are to investigate the mRNAs that bind to the FTO protein in the white fat tissue from mice. Methods: 1. White fat from CAG promoter driven transgenic Flag-tagged FTO mice were extracted with RNA protected. 2. Flag-FTO proteins were immunoprecipitated with control IP using IgG. 3. The immunoprecipitated RNAs were deep sequenced and analyzed. Results: We focused on the mRNAs which have functions related to lipid metabolism and homeostasis.

ORGANISM(S): Mus musculus

PROVIDER: GSE74255 | GEO | 2016/01/05

SECONDARY ACCESSION(S): PRJNA299573

REPOSITORIES: GEO

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