Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

HESC MIXL1+ MIXL1- microarray

ABSTRACT: Microarray analysis of isolated hES cells from day 3 of cardiac differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes. We identified 6,757 differentially regulated genes, of which 2,520 were upregulated ≥2-fold in the eGFP+ (MIXL1+) mesoderm population

ORGANISM(S): Homo sapiens

PROVIDER: GSE74664 | GEO | 2015/11/05

SECONDARY ACCESSION(S): PRJNA301126

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

hESC MIXL1+ MIXL1- microarray

Project description:Microarray analysis of isolated hES cells from day 3 of cardiac differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes. We identified 6,757 differentially regulated genes, of which 2,520 were upregulated â¥2-fold in the eGFP+ (MIXL1+) mesoderm population A stencil differentiation protocol was used to isolate mesodermal cells based on GFP expression from the MIXL1 locus. Microarray analysis of isolated cells from day 3 of differentiation was used to identify differences between MIXL1eGFP+ and MIXL1eGFP- transcriptomes.

2015-11-05 | E-GEOD-74664 | biostudies-arrayexpress

Change in Expression in U937 under MIXL1 enforced overexpression

Project description:While the role of the paired-type homeobox transcription factor MIXL1 in early embryonic development has been previously established, very little is known about the role of MIXL1 in adult hematopoiesis. To determine what role MIXL1 plays in adult hematopoietic cells, we established an enforced expression model for MIXL1 in the cell line U937, then analyzed the changes of gene expression caused by increased expression of MIXL1 by microarray analysis.

2016-11-21 | GSE52622 | GEO

Identification of targetted gene loci by MIXL1 in the AML cell line U937

Project description:While the role of the paired-type homeobox transcription factor MIXL1 in early ebryonic development has been previously established, very little is known about the role of MIXL1 in adult hematopoiesis. To determine what role MIXL1 plays in adult hematopoietic cells, we used an established enforced expression line for FLAG-tagged MIXL1 in U937, then isolated targetted regions of the genome by ChIP-Seq. Through this analysis, we identified new putative direct transcirptional targets for MIXL1 in AML and hematopoiesis.

2014-12-31 | GSE52781 | GEO

Single-cell RNAseq of Mixl1 knockout chimeric mouse embryos at embryonic day 8.5 of mouse development.

Project description:Chimeric embryos were generated to investigate the effect of Mixl1 knockout in mouse embryos by single-cell RNA-sequencing. Mixl1 is an essential transcription factor for mesendoderm development. Chimeric embryos contain tissue that is Mixl1+/+, which prevents global developmental failures. Embryos were generated by blastocyst injection of tdTomato-labelled, Mixl1-/- mouse embryonic stem cells into wild type embryos. After blastocyst harvest, cells were flow-sorted before 10X Genomics library preparation and single-cell RNA-sequencing.

2023-10-26 | E-MTAB-13409 | biostudies-arrayexpress

Transcriptomic changes in mouse Epiblast stem cells induced with Mixl1

Project description:We performed total RNA-seq in a epiblast stem cell model of mouse gastrulation to determine the effect of Mixl1 knockout and induction.

2024-07-27 | GSE161165 | GEO

Chromatin accessibility in mouse Epiblast stem cells induced with Mixl1

Project description:We performed ATAC-seq in a epiblast stem cell model of mouse gastrulation to determine the effect of Mixl1 knockout and induction on chromatin accessibility.

2024-07-27 | GSE161163 | GEO

Chromatin immunoprecipitation in mouse Epiblast stem cells induced with FLAG-Mixl1

Project description:We performed ChIP-seq in a epiblast stem cell model of mouse gastrulation to identify the genomic binding locations of mouse MIXL1

2024-07-27 | GSE161164 | GEO

Genome-wide profiling of Ta, Tbx16 and Mixl1 binding in early zebrafish embryos

Project description:Identification of Ta and Tbx16 binding sites during gastrulation, and Mixl1 binding sites during endoderm specification

2017-06-11 | GSE84612 | GEO

Gene expression analysis of hESC-derived-MESP1 expressing progenitors

Project description:A hESC MESP1-MCHERRY reporter line was used to isolate and study the molecular character of MESP1 expressing pre-cardiac progenitors, derived from hESC. MESP1 is a key-transcription factor for pre-cardiac mesoderm and is marking the progenitor for almost all cells of the heart. This reporter line was used to study cardiac differentiation and the derivation of early cardiac progenitors in vitro. hESCs were differentiated towards the cardiac lineage, expressing MESP1-mCherry at day 3 of differentiation. Total RNA obtained from isolated MESP1-mCherry expressing progenitors was compared to that of non-MESP1-expressing progenitors and undifferentiated hESCs in order to characterize MESP1-specific transcription factors and proteins.

2014-09-01 | E-GEOD-56721 | biostudies-arrayexpress

Gene expression analysis of hESC-derived-MESP1 expressing progenitors

2014-09-01 | GSE56721 | GEO