Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Whole genome survey of 32 Human Tissues

ABSTRACT: The Applied Biosystems Tissue Gene Expression Database, known as the Human Body Map, is a collection of gene expression profiles from 31 normal tissues and a Universal Human Reference RNA (Stratagene). The Human BodyMap was created using the Applied Biosystems Expression Array System. There were three replicates per tissue for a total of 96 microarrays. Keywords: Human tissue whole genome survey

ORGANISM(S): Homo sapiens

PROVIDER: GSE7905 | GEO | 2007/06/04

SECONDARY ACCESSION(S): PRJNA100079

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

The Applied Biosystems Tissue Gene Expression Database - Human Body Map. A whole genome survey of 32 Human Tissues

Project description:The Applied Biosystems Tissue Gene Expression Database, known as the Human Body Map, is a collection of gene expression profiles from 31 normal tissues and a Universal Human Reference RNA (Stratagene). The Human BodyMap was created using the Applied Biosystems Expression Array System. There were three replicates per tissue for a total of 96 microarrays. Keywords: Human tissue whole genome survey 31 human tissues from Clontech and 1 from Stratagene (UHR) were used to create the Applied Biosystems Human BodyMap (a.k.a.Tissue Gene Expression Database ). Digoxigenin-UTP-labeled cRNA was generated and linearly amplified from 1 µg of total RNA with three replicates per tissue, using the Applied Biosystems Chemiluminescent RT-IVT Labeling Kit v 2.0 and kit protocol. Microarray hybridization, chemiluminescence detection, image acquisition, and analysis were performed on the on the Applied Biosystems 1700 Chemiluminescent Microarray Analyzer using the Applied Biosystems Chemiluminescence Detection Kit and kit protocol. This dataset is part of the TransQST collection.

2007-06-04 | E-GEOD-7905 | biostudies-arrayexpress

Whole genome survey of the obesity model lean congenic mice B6.C-D7Mit353

Project description:We have analyzed the differential expression between the obesity model lean congenic B6.C-D7Mit353 (1) and the background C57BL/6J strains, in four different tissues of male mice: Brain, GWAT (Gonadal White Adipose Tissue), liver and muscle (gastrocnemius). From the 32,381 mouse genes covered by the Applied Biosystems AB1700 microarray system, we identified 330 genes passing FDR at 5% and 188 genes passing FDR at 1%, in any one of the four tissues examined. None of the genes that passed FDR testing were in the congenic donor region. 65 genes from the 8 megabases congenic region were assayed; only six genes (Thrsp, Kctd14, Myo7a, Capn5, Dgat2 and Mtap6) passed statistical significance filtering but not FDR filtering. (1) Diament & Warden 2004: PMID 14569280. Keywords: Congenic mice whole genome gene expression survey

2007-12-01 | GSE7431 | GEO

Homo sapiens

Project description:Wilms tumor versus Stratagene Universal Reference

| PRJNA87293 | ENA

Identify the dysregulated miRNA in human colorectal cancer tissues

Project description:Dysregulated miRNA in human colorectal cancer (CRC) were identified through comparison between 4 CRC tumors and their adjacent normal tissues by miRNA array. Histologically-confirmed CRC were included in this study. CRC tissues and paired adjacent normal tissues were obtained from the resected surgical specimens. The adjacent normal tissue is composed of normal colonic mucosa located at approximately 10 cm away from the cancer tissue. miRNA profiling of 754 human miRNAs was performed using TaqMan Human MiRNA Array Set v3.0. Quantitative real-time polymerase chain reaction (Q-PCR) was performed using Applied Biosystems 7900HT Real-Time PCR System (Applied Biosystems). Results were analyzed by the SDS RQ Manager 1.2 software (Applied Biosystems).

2013-03-20 | GSE45349 | GEO

Stratagene Universal Human RNA Hybridized to Affymetrix U133 Plus 2.0 and U133 A Chips

Project description:Stratagene universal human RNA hybridized to both U133 Plus 2.0 and U133 A arrays. Keywords: Affymetrix platform comparison

2006-07-25 | GSE3061 | GEO

Mouse Wild Type Tissue Survey

Project description:Survey of gene expression in 9 types of C57/Bl mouse wild type tissue.

2011-02-17 | GSE27346 | GEO

Tumor and Non Tumor Associated Skin Fibroblasts

Project description:Fibroblast cells were cultured from human skin, either tumor (basal cell carcinoma) or non-tumor tissue. Cells were propagated for several passages in culture, then switched to low (0.1%) serum conditions for 48 hours prior to mRNA harvest. Reference for all arrays was Stratagene Universal Human Reference RNA. Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set

2006-10-10 | GSE5951 | GEO

Angiogenesis gene expression upon Notch activation in human vascular smooth muscle cells

Project description:VSMCs were cultured on Jag1 or IgG-coated plates as described above. RNA was extracted using RNA isolation kit (Qiagen). Reverse-transcription PCR was done with RNA-to-cDNA kit (Applied Biosystems). cDNA was run on a TaqMan Low-Density Array, human angiogenesis panel (Applied Biosystems , Cat#4378725), amplified on a 7900 HT Fast Real Time PCR system (Applied Biosystems) according to manufacturer’s instructions.

2011-12-01 | GSE34024 | GEO

miRNA expression in human chondrocyte subtypes

Project description:Frozen blocks of human embryonic limb tissue were sectioned and laser capture microdissected to collect three human chodrocyte tissues - precursor, differentiated and hypertrophic chondrocytes.Total RNA including miRNAs was isolated and analyzed using Applied Biosystems OpenArray® Real-Time PCR system.

2013-10-23 | GSE49152 | GEO

Wilms tumor versus Stratagene Universal Reference

Project description:Gene expression comparison of Wilms tumor RNA versus Stratagene Universal reference RNA Keywords: repeat sample

2004-12-31 | GSE1158 | GEO