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Comparison of transcriptomes of mouse cumulus cells collected from mice before and after 6-h of hCG priming.

ABSTRACT: We sought to identify genes that are regulated by the ovulatory signals in mouse cumulus cells. We compared the transcriptomes between cumulus cells colected from mice before (hCG-0h) and after 6 h (hCG-6h) of hCG injection.

ORGANISM(S): Mus musculus

PROVIDER: GSE80255 | GEO | 2016/08/01

SECONDARY ACCESSION(S): PRJNA318400

REPOSITORIES: GEO

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Comparison of transcriptomes of mouse cumulus cells collected from mice before and after 6-h of hCG priming.

Project description:We sought to identify genes that are regulated by the ovulatory signals in mouse cumulus cells. We compared the transcriptomes between cumulus cells colected from mice before (hCG-0h) and after 6 h (hCG-6h) of hCG injection. 3 individual cumulus cell samples of hCG-0h and hCG-6h were collected and subjected to microarray study. Copmarison was done between hCG-0h and hCG-6h groups.

2016-08-01 | E-GEOD-80255 | biostudies-arrayexpress

Rhesus monkey cumulus cells revert to a mural granulosa cell state following an ovulatory stimulus

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Rhesus monkey cumulus cells revert to a mural granulosa cell state following an ovulatory stimulus

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Project description:Cumulus-oocyte complexes were isolated a seperate time-points to generate temporal complexes. Targets from two biological replicates at each time point (0h, 8h, 16h post-hCG treatment) were generated and the expression profiles were determined using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. Comparisons between the sample groups allow the identification of genes with temporal expression patterns. Keywords: time course

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Transcription profiling of cumulus cell oocyte complexes isolated at different time points of ovulation to identify differentially expressed genes.

Project description:Cumulus-oocyte complexes were isolated at seperate time-points to generate temporal complexes. Targets from two biological replicates at each time point (0h, 8h, 16h post-hCG treatment) were generated and the expression profiles were determined using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays. Comparisons between the sample groups allow the identification of genes with temporal expression patterns. Experiment Overall Design: 2 eCG-primed (48h) pooled cumulus-oocyte complexes, 2 eCG-primed (48h) hCG-treated (8h) pooled cumulus-oocyte complexes, and 2 eCG-primed (48h) hCG-treated (16h) pooled cumulus-oocyte complexes replicates were analyzed

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