Project description:Analysis of the different genotypes of non-tumorous mouse livers in 3 different ages revealed more genetic alterations in Krt18-/- and Krt18+/- compared to wt livers. This speaks in favor of a higher genomic instability being causally related to K-deficiency and aging, even at a preneoplastic stage.
Project description:Analysis of the different genotypes of non-tumorous mouse livers in 3 different ages revealed more genetic alterations in Krt18-/- and Krt18+/- compared to wt livers. This speaks in favor of a higher genomic instability being causally related to K-deficiency and aging, even at a preneoplastic stage.
Project description:Analysis of the different genotype tumors revealed enhanced chromosomal aberrations in all tumors. The most chromosomal aberrations were found in the krt18-/- tumors.
Project description:Analysis of the different genotype tumors revealed enhanced chromosomal aberrations in all tumors. The most chromosomal aberrations were found in the krt18-/- tumors. Two-condition experiment: wt (non-affected) liver tissue vs. wt tumor / krt18+/-tumor / krt18-/- tumor
Project description:A prospective study was performed on neurosurgical samples from 18 patients to evaluate the use of full-field optical coherence tomography (FF-OCT) in brain tumor diagnosis. FF-OCT captures en face slices of tissue samples at 1 ?m resolution in 3D to a penetration depth of around 200 ?m. A 1 cm(2) specimen is scanned at a single depth and processed in about 5 min. This rapid imaging process is non-invasive and requires neither contrast agent injection nor tissue preparation, which makes it particularly well suited to medical imaging applications. Temporal chronic epileptic parenchyma and brain tumors such as meningiomas, low-grade and high-grade gliomas, and choroid plexus papilloma were imaged. A subpopulation of neurons, myelin fibers and CNS vasculature were clearly identified. Cortex could be discriminated from white matter, but individual glial cells such as astrocytes (normal or reactive) or oligodendrocytes were not observable. This study reports for the first time on the feasibility of using FF-OCT in a real-time manner as a label-free non-invasive imaging technique in an intraoperative neurosurgical clinical setting to assess tumorous glial and epileptic margins.
Project description:This SuperSeries is composed of the following subset Series: GSE23352: Whole-genome gene expression profiles of non-tumorous human lung tissues: Laval set GSE23529: Whole-genome gene expression profiles of non-tumorous human lung tissues: UBC set GSE23545: Whole-genome gene expression profiles of non-tumorous human lung tissues: GRNG set Refer to individual Series
Project description:Transcriptional silencing of tumor suppressor genes and other cancer-related genes induced by promoter CpG island hypermethylation is an important epigenetic mechanism of hepatocarcinogenesis. Previous studies have established methylation profiles of hepatocellular carcinomas (HCCs) and demonstrated that methylation of several candidate genes in resected tissues may be associated with time to recurrence. The goals of our study were to test whether specific promoter methylation and mRNA levels of candidate genes, as well as global changes in DNA methylation, can be linked with time to recurrence and clinicopathological variables in a homogenous study group of HCC patients. Forty-three tumorous and 45 non-tumorous liver tissue samples from the surgical margin were obtained from HCV-positive, HBV-negative HCC patients who underwent tumor resection surgery and who were monitored for tumor recurrence thereafter (median follow-up time: 16 months (range, 0-79 months)). Methylation-specific PCR was used to assess the promoter methylation status of P16(INK4a), SOCS-1, RASSF1A, APC, GSTP1, RIZ1, and MGMT genes, while the level of LINE-1 methylation was used as marker of global DNA methylation levels. Methylation frequencies in P16(INK4a), RASSF1A, APC, GSTP1, and RIZ1 genes were significantly greater in tumorous versus non-tumorous tissues. Methylation of RIZ1 in non-tumorous tissues was significantly associated with time to recurrence. Additionally, genomic DNA was significantly more hypomethylated in tumorous tissues, and this change was associated with shorter recurrence, but not with clinicopathological features. In conclusion, this study supports the role of aberrant methylation in the pathobiology of HCV-positive HCCs. The finding that RIZ1 methylation and increased levels of LINE-1 hypomethylation in non-tumorous tissues are associated with time to recurrence underscores the importance of assessing the epigenetic state of the liver remnant.