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Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE83409 | GEO | 2016/12/05

SECONDARY ACCESSION(S): PRJNA325821

REPOSITORIES: GEO

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Chromatin binding of Gcn5 in Drosophila is largely mediated by CP190.

Project description:Centrosomal 190 kDa protein (CP190) is a promoter binding factor, mediates long-range interactions in the context of enhancer-promoter contacts and in chromosomal domain formation. All Drosophila insulator proteins bind CP190 suggesting a crucial role in insulator function. CP190 has major effects on chromatin, such as depletion of nucleosomes, high nucleosomal turnover and prevention of heterochromatin expansion. Here, we searched for enzymes, which might be involved in CP190 mediated chromatin changes. Eighty percent of the genomic binding sites of the histone acetyltransferase Gcn5 are colocalizing with CP190 binding. Depletion of CP190 reduces Gcn5 binding to chromatin. Binding dependency was further supported by Gcn5 mediated co-precipitation of CP190. Gcn5 is known to activate transcription by histone acetylation. We used the dCas9 system to target CP190 or Gcn5 to a Polycomb repressed and H3K27me3 marked gene locus. Both, CP190 as well as Gcn5, activate this locus, thus supporting the model that CP190 recruits Gcn5 and thereby activates chromatin.

| S-EPMC5389606 | biostudies-literature

Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [ChIP-seq]

Project description:80% of the genomic binding sites of the histone acetyltransferase Gcn5 are colocalizing with CP190 binding. Depletion of CP190 reduces the number of Gcn5 binding sites and binding strength to chromatin. Binding dependency was further supported by Gcn5 mediated co-precipitation of CP190

2016-12-05 | GSE83408 | GEO

Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190 [RNA-seq]

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Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190

Project description:Chromatin Binding of Gcn5 in Drosophila is Largely Mediated by CP190

| PRJNA325821 | ENA

Differential Chromatin Binding of the Drosophila Dosage Compensation Complex

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2012-12-11 | E-GEOD-37865 | biostudies-arrayexpress

Topological screen identifies hundreds of Cp190 and CTCF dependent Drosophila chromatin insulator elements

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-07-19 | GSE198763 | GEO

EAST Organizes Drosophila Insulator Proteins in the Interchromosomal Nuclear Compartment and Modulates CP190 Binding to Chromatin.

Project description:Recent data suggest that insulators organize chromatin architecture in the nucleus. The best studied Drosophila insulator proteins, dCTCF (a homolog of the vertebrate insulator protein CTCF) and Su(Hw), are DNA-binding zinc finger proteins. Different isoforms of the BTB-containing protein Mod(mdg4) interact with Su(Hw) and dCTCF. The CP190 protein is a cofactor for the dCTCF and Su(Hw) insulators. CP190 is required for the functional activity of insulator proteins and is involved in the aggregation of the insulator proteins into specific structures named nuclear speckles. Here, we have shown that the nuclear distribution of CP190 is dependent on the level of EAST protein, an essential component of the interchromatin compartment. EAST interacts with CP190 and Mod(mdg4)-67.2 proteins in vitro and in vivo. Over-expression of EAST in S2 cells leads to an extrusion of the CP190 from the insulator bodies containing Su(Hw), Mod(mdg4)-67.2, and dCTCF. In consistent with the role of the insulator bodies in assembly of protein complexes, EAST over-expression led to a striking decrease of the CP190 binding with the dCTCF and Su(Hw) dependent insulators and promoters. These results suggest that EAST is involved in the regulation of CP190 nuclear localization.

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Gcn5-mediated histone acetylation governs nucleosome dynamics in spermiogenesis

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-11-07 | GSE134561 | GEO

Kc cells from Drosophila melanogaster during ecdysone treatment and CP190 knockdown

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-10-14 | GSE30741 | GEO

Differential Chromatin Binding of the Drosophila Dosage Compensation Complex

Project description:This SuperSeries is composed of the SubSeries listed below.

2012-12-11 | GSE37865 | GEO