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Silencing miR-31 in naive CD4+ T cells by antagomiRNA

ABSTRACT: To describe the global profile of genes regulated by miR-31, we compared the transcriptomics between miR-31 silenced and antagomir control treated naive CD4+ T cells. Interestingly, the cells could be separated into two subsets according FCS and SSC by flow cytometer after the silence of miR-31, one (~65%) was small sized cells with low granularity, the other (~30%) was large cells with high granularity, whereas the antagoNC treated naïve CD4+ T cells only contain a marginal fraction of large cells. We pooled “antagoNC”, “antagomiR31S” and “antagomiR31L” naïve CD4+ T cells from 5 healthy donors, respectively, and analyzed the differential genes among those three subsets.

ORGANISM(S): Homo sapiens

PROVIDER: GSE83853 | GEO | 2022/12/24

REPOSITORIES: GEO

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