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In vitro B cell activation by Th1 cells promotes the transcriptional programming of antibody-secreting cells, while B cell activation in presence of Th2 cells maintains the B cell identity transcriptional program

ABSTRACT: To investigate the role of Th1 versus Th2 cell help on B cell activation and differentiation into B cell effectors and antibody secreting cells (ASCs), we utilized an in vitro culture system to activate B cells in the presence of Th1 or Th2 polarized CD4+ T cells. To obtain an unbiased mRNA profile of these cells, we performed a kinetic mRNA profiling analysis on Be1 cells (B cells cultured with Th1 cells) or Be2 cells (B cells cultured with Th2 cells) on days 1, 2, 3, and 4 of co-culture.

ORGANISM(S): Mus musculus

PROVIDER: GSE84948 | GEO | 2019/05/22

REPOSITORIES: GEO

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