Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Time course gene expression of HUVEC after TNF-alpha treatment

ABSTRACT: The many steps involved in the production of a mature mammalian mRNA are extensively coupled, and levels of both precursors and products can be measured using expression and genomic tiling microarrays. Different probes in these arrays targeting the same transcript often give different signals; then, precursor (nascent) RNA – which is present transiently at low concentrations – is difficult to detect. Keywords: TNFa stimulation time course of HUVEC

ORGANISM(S): Homo sapiens

PROVIDER: GSE9055 | GEO | 2008/09/13

SECONDARY ACCESSION(S): PRJNA102567

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of human umbilicial vein endothelial cells after TNF-alpha treatment

Project description:The many steps involved in the production of a mature mammalian mRNA are extensively coupled, and levels of both precursors and products can be measured using expression and genomic tiling microarrays. Different probes in these arrays targeting the same transcript often give different signals; then, precursor (nascent) RNA â which is present transiently at low concentrations â is difficult to detect. Experiment Overall Design: Using tumor necrosis factor a (TNF-alpha) to switch on transcription of selected human genes rapidly and synchronously, we apply samples collected every 15 min to arrays

2008-10-25 | E-GEOD-9055 | biostudies-arrayexpress

HUVEC gene profile after TNF-stimulation

Project description:HUVEC were left untreated or stimulated for 5h with 2 ng/ml TNF. Comparsion of the gene profiles revealed TNF-mediated gene expression changes in HUVEC. Keywords: parallel sample

2005-05-12 | GSE2639 | GEO

The effect of TNFa on endothelial cells

Project description:To understand the transcripts induced by TNFa and how these may interact in inflammatory networks, the transcriptome of HUCECs stimulated with TNFa was examined in 3 different pools each cosisting of 10 different HUVEC isolates. In the study present here, HUVEC isolates from 3 pools of 10 different individuals were cultured until passage 4 in fully supplemented growth conditions. Passage 4 HUVEC isolates were then treated with TNFa (10ng/ml), with RNA extracted at time points 0, 1, 1.5, 2, 4, 5, and 6hrs after TNFa treatment . RNA was extracted and hybridised onto CodeLink microarrays.

2011-11-29 | E-GEOD-27870 | biostudies-arrayexpress

The effect of TNFa on endothelial cells

2011-11-29 | GSE27870 | GEO

The gene expression of HUVEC in response to the cytokine stimulations

Project description:The response of endothelial cells to the tissue environment has a critical impact on their function in inflammation and tumors. In particular, cytokine stimulation such as TNFa, IL-4, IFNg, and LTbR induces gene expression changes, which significantly impact endothelial cell function and differentiation. To understand the degree of gene expression change, we performed a transcriptome analysis of HUVEC.

2024-05-01 | GSE254958 | GEO

Transcription profiling by array of human umbilical vein endothelial cells (HUVEC) stimulated with TNF vs. controls reveals TNF induces distinct expression programs

Project description:HUVEC were left untreated or stimulated for 5h with 2 ng/ml TNF. Comparsion of the gene profiles revealed TNF-mediated gene expression changes in HUVEC.

2007-11-01 | E-GEOD-2639 | biostudies-arrayexpress

HUVEC T Cell Irradiation Study

Project description:Question Addresses: What is the gene expression profile from human umbilical vein endothelial cells (HUVEC) and human Jurkat T cells after irradiation (IR)? What, if any, is the effect of co-culturing these two cell types on gene expression? There are eight experimental conditions for this experiment: (1) non-irradiated HUVEC; (2) irradiated HUVEC; (3) non-irradiated Jurkat; (4) irradiated Jurkat; (5) non-irradiated HUVEC + non-irradiated Jurkat+; (6) non-irradiated HUVEC + irradiated Jurkat; (7) irradiated HUVEC + non-irradiated Jurkat; (8) irradiated HUVEC + irradiated Jurkat. A common, pooled reference consisting of RNA taken from conditions 1-8 as described above was used for all hybridizations.

2022-06-03 | GSE39019 | GEO

Confluent versus Sub-confluent HUVEC

Project description:HUVEC (N=3 isolates) were separately grown to sub-confluency, and to confluency. In order to study the effects of growth and contact inhibition on the transcriptome, the microarray gene expression profiles of these sub-confluent and confluent HUVEC were compared. Keywords: HUVEC, confluency, contact inhibition

2007-12-01 | GSE9334 | GEO