Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cytotoxic effects of radiocontrast dyes on human umbilical cord mesenchymal stem cells

ABSTRACT: Discography is an imaging procedure typically performed on patients who report extended periods of pain.Usually radiocontrast agents are used to diagnose the discogenic low back pain. This study was focused on investigating the effects of two clinically used, non-ionic radiocontrast dyes, isovue-370 and omnipaque-240, on the human umbilical cord mesenchymal stem cells (UC-MSCs). Human UC-MSCs were cultured and treated with various concentrations of Omnipaque and Isovue for 24 hr. The cell growth was inhibited by both the dyes. The results showed that both the dyes significantly affected the cell morphology and viability as well as caused apoptosis in UC-MSCs. The global gene expression analysis showed that the most effected genes were those involved in the apoptosis, DNA damage and toxicity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE96043 | GEO | 2017/10/04

SECONDARY ACCESSION(S): PRJNA378653

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Differential gene expression profiles induced by Hoechst dyes

Project description:Bisbenzimides, or Hoechst 33258 (H258), and its derivative Hoechst 33342 (H342) are archetypal molecules for designing minor groove binders, and widely used as tools for staining DNA and analyzing side population cells. They are supravital DNA minor groove binders with AT selectivity. H342 and H258 share similar biological effects based on the similarity of their chemical structures, but also have their unique biological effects. For example, H342, but not H258, is a potent apoptotic inducer and both H342 and H258 can induce transgene overexpression in in vitro studies. However, the molecular mechanisms by which Hoechst dyes induce apoptosis and enhance transgene overexpression are unclear. The purpose of the present study is to determine the molecular mechanisms underlying different biological effects between H342 and H258.

2011-04-16 | GSE28616 | GEO

Differential gene expression profiles induced by Hoechst dyes

2011-04-16 | E-GEOD-28616 | biostudies-arrayexpress

Transcriptomic regulation of human mesenchymal stem cells in delivering anti-tumorigenic effects on leukaemic cell lines

Project description:The present study was designed to investigate the effect of human umbilical cord-derived MSCs (UC-MSCs) on the proliferation of leukaemic cells and gauged the transcriptomic modulation and the signalling pathways that potentially inflicted by UC-MSCs.

2020-09-15 | GSE116677 | GEO

Mesenchymal Stem Cells (MSCs) Alleviate Experimental Immune-mediated Liver Injury via Chitinase 3-like protein 1-mediated T cell suppression

Project description:RNA-seq for MSCs from human bone marrow (BM-MSCs), olfactory mucosa (OM-MSCs), and umbilical cord (UC-MSCs) (2 donors for each). RNA-sequencing for human naïve CD3+ T cells, CD3+ T cells activated with PHA (2.5 μg/mL) for 48 h， and CD3+ T cells co-cultured with human UC-MSCs in the presence of PHA for 48 h.

2021-02-09 | GSE166327 | GEO

Transcriptional single cell analysis of human umbilical cord derived mesenchymal stem cells

Project description:Mesenchymal stem cells (MSCs) are a population of multipotent cells with an attractive ability to promote tissue repair by regulating regeneration and inflammation. Ironically, the immune regulatory capacity of MSCs, regardless of their tissue origin, is endowed by inflammatory cytokines (e.g., IFNg plus TNFa). Effective application of MSCs relies on the production of relatively homogeneous MSCs. However, the cellular heterogeneity, and the differentiation trajectories of in vitro expanded MSCs remains unclear. We profiled the transcriptomes of 361 single MSCs derived from two umbilical cords (UC-MSCs). The MSCs are from different passages and culture conditions with or without inflammatory cytokine stimulation. Weighted gene correlation network analysis for the expression of their highly variable genes revealed that UC-MSCs surprisingly possess only limited heterogeneity. Cell cycle based principal component analysis showed that the limited heterogeneity identified in these UC-MSCs was strongly associated with their entrance into the G2/M phase. This was further proven by the observation that one featured gene CD168 was expressed in a cell-cycle dependent manner. When CD168high UC-MSCs were sorted and cultured in vitro, they repopulated into a new community that is similar to the original status. Our results demonstrated that in vitro expanded UC-MSCs is a well-organized population with limited heterogeneity dominated by cell cycle status. Thus, our studies argues that standardization of MSCs for disease treatment is possible.

2019-05-15 | GSE117837 | GEO

Effects of human adipose tissue-derived and umbilical cord tissue-derived mesenchymal stem cells in a dextran sulfate sodium-induced mouse model

Project description:Background: Mesenchymal stem cells (MSCs) can be acquired from medical waste. MSCs are easily expanded and have multiple functions, including anti-inflammatory effects. We evaluated the effects of human adipose tissue-derived MSCs (AD-MSCs) and umbilical cord tissue-derived MSCs (UC-MSC) in a dextran sulfate sodium (DSS)-induced mouse model. Methods: Human AD-MSCs and UC-MSCs (1 × 106 cells) were injected intravenously into a 7-day DSS-induced colitis model. The therapeutic effects of cell origin, injection timing, and supernatants obtained from MSC cultures were evaluated. We also analyzed mRNA expression in MSCs, tissues, and intestinal flora. Results: AD-MSCs and UC-MSCs had strong anti-inflammatory effects when injected on day 3 in a mouse model. On day 11, mRNA levels of inflammatory factors in colon tissues were significantly decreased after injection of MSCs on day 3. Supernatants from MSCs culture decreased mRNA levels of tumor necrosis factor (Tnf)-α, but had reduced therapeutic effects compared with MSC cell injection. RNA sequencing using colon tissues obtained the day after cell injection revealed changes in the TNF-α/nuclear factor-κB and T-cell receptor signaling pathways. Additional analyses showed that several factors, including chromosome 10 open reading frame 54, stanniocalcin-1, and TNF receptor superfamily member 11b were increased in MSCs after adding serum from DSS colitis mice. Furthermore, both AD-MSCs and UC-MSCs maintained the balance of intestinal flora. Conclusion: AD-MSCs and UC-MSCs showed therapeutic effects against inflammation after early cell injection while maintaining the intestinal flora. Although supernatants showed therapeutic effects, cell injection was more effective against inflammation.

2019-09-11 | GSE137173 | GEO

Effects of human adipose tissue-derived and umbilical cord tissue-derived mesenchymal stem cells in a dextran sulfate sodium-induced mouse model

2019-11-19 | GSE136397 | GEO

LRP1high subpopulation of human umbilical cord mesenchymal stem cell (hUC-MSC)transplantationimproves ovarian function in POF and aging mice.

Project description:This study revealed the heterogeneity of UC-MSCs using single-cell sequencing technology (10x Genomics). Through in vitro and in vivo experiments, it was determined that the LRP1high UC-MSCs subpopulation has therapeutic effects in improving ovarian function. This finding provides theoretical support for the screening of stem cell preparations for use in POF, aiming to reduce the impact of other interfering cell components on ovarian tissue and provide precise, safe, and effective clinical strategies for stem cell therapy in POF. Mechanistic exploration further revealed that the LRP1high UC-MSCs subpopulation primarily improves mitochondrial function of granulosa cells by modulating DNA damage pathways, extracellular matrix-related signals, and cellular metabolism. Consequently, it more effectively enhances the quality of aging mouse oocytes, offering new insights into targeting LRP1high UC-MSCs for precise treatment of premature ovarian failure.

2024-03-13 | GSE240185 | GEO

LRP1high subpopulation of human umbilical cord mesenchymal stem cell (hUC-MSC)transplantationimproves ovarian function in POF and aging mice

2024-03-13 | GSE239500 | GEO

Development of gene expression signatures for IGF-1 overexpressing human umbilical cord-derived mesenchymal stem cells

Project description:As a key factor for differentiation and development, IGF-1 has attracted scientists’ attention gradually in recent years. However, its exact effects in human umbilical cord-derived mesenchymal stem cells (UC-MSCs) are still unclear. In this study, we mainly investigated whether IGF-1 could affect some biological functions of UC-MSCs.

2015-10-06 | GSE73724 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data