Project description:Cheese samples analyzed using SPME GC/MS. Headspace sampled at 160C with a Polydimethylsiloxane / Divinylbenzene (PDMS/DVB) df 65 um fiber. No prior sample processing or preparation have been carried out.
Project description:Volatiles from had space of the cultures of fungus, bacteria and interacting bacteria and fungus. The volatiles are collected from headspace with Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um SPME.
Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).
Project description:The GC-MS analysis conducted via headspace sampling with a Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um Solid Phase Microextraction (SPME) fiber. A Thermo Scientific Trace 1310 GC (TG-5ms 5 30m length, 0.25mm ID, 0.25 �m film thickness column) and a TSQ 8000 EVO mass spectrometer are used. Sputum samples for the WinCF model by RAQ.
Project description:Samples from extraction of volatile organic compounds from frogs using two sampling methods: in vivo and sampling from the skin and a DVB/CAR/PDMS-SPME fiber. Splitless injection mode using a desorption temperature of 250C. Separation was performed initiating to 40C for 3 min, and then increasing the temperature to 100C using a rate of 6C/min, afterwards incresing to 200C changing the rate to 4C/min, and finally increasing to 300C, using a rate of 20C/min, and maintaing to this temperature for 3 min. Analysis were performed in a GC HP 6890 Series equipped with an Agilent Mass Selective Detector 5973 (Agilent technologies, Palo Alto, CA, USA). Separation was performed on a BP-5 capillary GC column (30 m 0.25 mm 0.25 um, SGE, Austin, TX, USA) using helium as a carrier gas at a flow rate of 1.0 mL/min.
Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).
Project description:Untargeted metabolomics analysis of in vitro headspace volatiles from 81 Pseudomonas aeruginosa bacterial isolates from individuals with cystic fibrosis. Headspace volatiles were collected using solid-phase microextraction (SPME) (in triplicate) and comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry (GCxGC-TOFMS). 15 replicates of un-inoculated media were prepared and analyzed in parallel, for a total of 258 samples.
Project description:IL-17 Inhibition in Spondyloarthritis Associates with Subclinical Gut Microbiome Perturbations and a Distinctive IL-25-Driven Intestinal Inflammation
Volatile short-chain fatty acids analysis.
Approximately 50 mg of sample were placed in 2 ml borosilicate vials capped with a screw cap with silicone septum. The capped vials were stored at -20 C and thawed immediately prior to analysis. The GC-MS analysis was carried out using the Agilent 7200 GC/QTOF equipped with robotic sampler system. The volatiles from the sample were extracted from headspace using Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um Solid Phase Microextraction Fiber (SPME) for 30 minutes at 50 C. The fiber was then inserted into the injector equipped with Merlin septum heated to 250 C and the adsorbed compounds were desorbed for 1 minute.
Project description:Samples from extraction of volatile organic compounds from Malassezia species growing in solid mDixon media using DVB/CAR/PDMS-SPME fiber. Splitless injection mode using a desorption temperature of 250C. Analysis were performed in a GC HP 6890 Series equipped with an Agilent Mass Selective Detector 5973 (Agilent technologies, Palo Alto, CA, USA). Separation was performed on a BP-5 capillary GC column (30 m 0.25 mm 0.25 um, SGE, Austin, TX, USA) using helium as a carrier gas at a flow rate of 1.1 mL/min.