Project description:Cheese samples analyzed using SPME GC/MS. Headspace sampled at 160C with a Polydimethylsiloxane / Divinylbenzene (PDMS/DVB) df 65 um fiber. No prior sample processing or preparation have been carried out.
Project description:This dataset comprises the raw mass spectrometry files generated from the analysis of VOCs extracted from the pulp of Theobroma grandiflorum (Copoazu) at three distinct maturation stages (ripe, overripe, and medium). The study used HS-SPME-GC-MS. A Divinylbenzene/carbon wide-range/polydimethylsiloxane (DVB/CWR/PDMS) 80 um x 10 mm fiber was used in combination with a Gas Chromatograph HP 6890 Series equipped with an Agilent Mass Selective Detector 5973. The VOCs were separated on a capillary GC column of 5% Phenyl/95% Dimethyl Polysiloxane (30 m x 0.25 mm x 0.25 um).
Project description:Volatiles from had space of the cultures of fungus, bacteria and interacting bacteria and fungus. The volatiles are collected from headspace with Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um SPME.
Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).
Project description:The GC-MS analysis conducted via headspace sampling with a Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um Solid Phase Microextraction (SPME) fiber. A Thermo Scientific Trace 1310 GC (TG-5ms 5 30m length, 0.25mm ID, 0.25 �m film thickness column) and a TSQ 8000 EVO mass spectrometer are used. Sputum samples for the WinCF model by RAQ.
Project description:Samples from extraction of volatile organic compounds from frogs using two sampling methods: in vivo and sampling from the skin and a DVB/CAR/PDMS-SPME fiber. Splitless injection mode using a desorption temperature of 250C. Separation was performed initiating to 40C for 3 min, and then increasing the temperature to 100C using a rate of 6C/min, afterwards incresing to 200C changing the rate to 4C/min, and finally increasing to 300C, using a rate of 20C/min, and maintaing to this temperature for 3 min. Analysis were performed in a GC HP 6890 Series equipped with an Agilent Mass Selective Detector 5973 (Agilent technologies, Palo Alto, CA, USA). Separation was performed on a BP-5 capillary GC column (30 m 0.25 mm 0.25 um, SGE, Austin, TX, USA) using helium as a carrier gas at a flow rate of 1.0 mL/min.
Project description:Skin samples collected from underarm w/ PDMS for 30 seconds. Samples used for optimization of GC headspace methodology wrt desorption time, cryofocusing, and size of PDMS patch (10mL vials were used).
Project description:Untargeted metabolomics analysis of in vitro headspace volatiles from 81 Pseudomonas aeruginosa bacterial isolates from individuals with cystic fibrosis. Headspace volatiles were collected using solid-phase microextraction (SPME) (in triplicate) and comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry (GCxGC-TOFMS). 15 replicates of un-inoculated media were prepared and analyzed in parallel, for a total of 258 samples.
Project description:IL-17 Inhibition in Spondyloarthritis Associates with Subclinical Gut Microbiome Perturbations and a Distinctive IL-25-Driven Intestinal Inflammation
Volatile short-chain fatty acids analysis.
Approximately 50 mg of sample were placed in 2 ml borosilicate vials capped with a screw cap with silicone septum. The capped vials were stored at -20 C and thawed immediately prior to analysis. The GC-MS analysis was carried out using the Agilent 7200 GC/QTOF equipped with robotic sampler system. The volatiles from the sample were extracted from headspace using Polydimethylsiloxane/Divinylbenzene (PDMS/DVB) df 65 um Solid Phase Microextraction Fiber (SPME) for 30 minutes at 50 C. The fiber was then inserted into the injector equipped with Merlin septum heated to 250 C and the adsorbed compounds were desorbed for 1 minute.