Project description:Human embryonic kidney (HEK) cells 293 are analyzed by two state-of-the-art MS instruments (Thermo Orbitrap Fusion and Q Exactive HF) in quadruplicate for precision evaluation.
Project description:ddMS2 run of mouse lung tissue and plasma extract using C8 column in 7.5-minute gradient and positive polarity mode in Q Exactive plus.
Project description:Using protein microarrays, derived from 642 His-tag proteins, we could distinguish sera from breast-nodule positive patients and healthy control individuals. Each Protein microarray was divided in to 4 sub-arrays. Each protein was spotted in duplicates in each sub-array. For evaluation 24 malignant, 16 benign breast cancer serum samples and 20 healthy control serum samples were used.