Metabolomics

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GNPS_198 root bacteria+interactions


ABSTRACT: Crude extracts of 198 root-associated bacteria, grown on TSB agar. Additionally, interaction zones of selected bacterial strains were extracted. All file names contain essential information about the sample, first the strain number of the strain collection, then the extraction solvent (E=ethyl acetate, M= methanol). Filename 690_E.mzxml would correspond to strain 690, grown on TSB, extracted with ethyl acetate. For measuring antibiotic interactions of two strains, halo zones of the agar were extracted the same way as for the wild type strains. Filenames depict first the antibioticall active strain, then the sensitive strain, and finally the extraction solvent. Filename 562480M.mzxml would thus correspond to strain 562 that was grown on a lawn of strain 480, its extraction zone was extracted with methanol.

INSTRUMENT(S): micrOTOF-Q II

ORGANISM(S): Root-associated Bacteria

SUBMITTER: Max Cruesemann  

PROVIDER: MSV000081381 | GNPS | Tue Jul 25 01:07:00 BST 2017

REPOSITORIES: GNPS

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Publications


Soil-dwelling microbes are the principal inoculum for the root microbiota, but our understanding of microbe-microbe interactions in microbiota establishment remains fragmentary. We tested 39,204 binary interbacterial interactions for inhibitory activities in vitro, allowing us to identify taxonomic signatures in bacterial inhibition profiles. Using genetic and metabolomic approaches, we identified the antimicrobial 2,4-diacetylphloroglucinol (DAPG) and the iron chelator pyoverdine as exometaboli  ...[more]

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