Project description:The serum samples from wild type mice fed high-fat diet for 12 weeks (WT_Serum) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (KI_Serum) were mixed separately, and subjected to proteomic study by Label-free quantitative techniques and mass spectrometry-based proteomics techniques in Jingjie PTM BioLab (Hangzhou) Co. Ltd (www.ptm-biolab.com.cn). The difference was determined by 1.5-fold-change criterion, FDR < 0.01.
Project description:The results provide information on the gene expression in dissected KrasG12D-driven lung tumor in mice. Mice were fed on high-calorie diet starting 3 months before tumor induction. Dissected tumors were obtained 11 weeks after tumor induction.
Project description:The epididymal adipose tissue (eWAT) samples from wild type mice fed high-fat diet for 12 weeks (H_WT_E) and Mdm2 adipocyte-specific knock-in mice fed high-fat diet for 12 weeks (H_KI_E) were mixed separately, and subjected to proteomic study by Label-free quantitative techniques and mass spectrometry-based proteomics techniques, etc. The proteomics of mixed eWAT samples were performed in Jingjie PTM BioLab (Hangzhou) Co. Ltd (www.ptm-biolab.com.cn). The difference was determined by 1.5-fold-change criterion, FDR < 0.01.
Project description:To examine the effects of antibiotics on gene expression in the prostate of a mouse model of prostate cancer fed a high-fat diet, we extracted RNA from the prostates of high-fat diet fed mice with or without antibiotics treatment, and compared their gene expressions with the two groups using microarrays.
Project description:Cardiomyocytes were isolated from the hearts of C57BL/6 mice or C57BL/6 mice that had been fed a high fat diet for 26 weeks, receiving an intraperitoneal injection of streptozotocin 12 weeks into the study. Cells were exposed to either linagliptin (9nmol/L) or vehicle (0.1% DMSO) for 24 hours before comparison of RNA levels by RNA-sequencing.
Project description:Purpose: To explore the influence of Ruminococcus torques treatment in mice intestinal epithelia. Method: Mice were pretreated with antibiotics for 3 days, then mice were fed with a high-fat diet and gavaged with PBS or R. torques (108 CFU/mouse) every 3 days for 2 weeks. mRNA profiles were generated by deep sequencing.Results: Several signaling pathways were changed after R. torques treatment, including response to hypoxia pathway. R. torques treatment significantly inhibits Epas1-mediated transcription effect.
Project description:There are 10 mice in the experiment, named REC. The mice were fed with high salt diets (5% NaCl) for 4 weeks and then fed with normal salt diets for 4 weeks. Then extracted DNA from mice gastric flora to detect changes in the gastric flora of mice.
Project description:The results provide information on the gene expression in dissected KrasG12D-driven lung tumor in mice. Mice were fed on high-calorie diet starting 3 months before tumor induction. Dissected tumors were obtained 11 weeks after tumor induction. Total RNA extracted from Kras-driven lung tumors of mice fed with standard diet or high calorie diet
Project description:Hepatic transcriptome of junctional adhesion molecule A knockout, F11r–/– mice fed a Western diet (WD) for eight weeks. A cohort of WD-fed mice were treated with IgG or α4β7 mAb for four weeks starting at week four following initiation of the WD.