GNPS - Lamellodysidea herbacea GUM007 (sponge) extract
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ABSTRACT: GUM007 whole-sponge tissue was pulverized and extracted with 3 x 20-ml MeOH for 30 to 60 min on a benchtop nutator. The combined extracts were dried in vacuo. Approx. 100 mg MeOH extract and 40 mL RNALater (used to store the sponge tissue) were loaded onto 1g C18 solid-phase extraction columns (Canadian Life Science) and fractionated from 5% - 100% MeCN. Three standards, dysinosin A, dysinosin B, and dysinosin C, kindly provided by Ron Quinn from Griffith University, were prepared as 0.1 mg/ml solutions in MeOH and analyzed with the GUM007 MeCN fractions using the Agilent 6530 Accurate-Mass Q-TOF MS with a Phenomenex Kinetex 5 uM C18(2) 100-A, 150 by 4.6 mm column (0 to 3 min 5% MeCN isocratic, 3 - 23 min, 5 - 100% MeCN, 23 to 26 min 100% MeCN isocratic at 0.7 ml/min). The dysinosin standards revealed a characteristic loss of sulfate, which led to the discovery of the desoxydysinosins, present in the 15% and 20% MeCN fractions.
INSTRUMENT(S): 6530 Q-TOF LC/MS (Agilent)
ORGANISM(S): Lamellodysidea Herbacea (ncbitaxon:279619)
SUBMITTER: Bradley S. Moore
PROVIDER: MSV000086050 | GNPS |
REPOSITORIES: GNPS
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