Project description:<p>One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </p><p><br></p><p>Cell culture sampling is reported in the current study.</p><p>Method development is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS826" target="_blank">MTBLS826</a></p><p>The effect of salt and water on metabolite detection is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS839" target="_blank">MTBLS839</a></p><p>Environmental sampling is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS844" target="_blank">MTBLS844</a></p><p>Method quantification is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS848" target="_blank">MTBLS848</a></p><p>Solid phase extraction is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS849" target="_blank">MTBLS849</a></p>
Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> Solid phase extraction is reported in the current study MTBLS849.</br> Method development is reported in MTBLS826.</br> The effect of salt and water on metabolite detection is reported in MTBLS839.</br> Cell culture sampling is reported in MTBLS843.</br> Environmental sampling is reported in MTBLS844.</br> Method quantification is reported in MTBLS848.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'><span class='label label-success'>MTBLS826</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'><span class='label label-success'>MTBLS839</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'><span class='label label-success'>MTBLS848</span></a>
Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> Method quantification is reported in the current study MTBLS848.</br> Method development is reported in MTBLS826.</br> The effect of salt and water on metabolite detection is reported in MTBLS839.</br> Cell culture sampling is reported in MTBLS843.</br> Environmental sampling is reported in MTBLS844.</br> Solid phase extraction is reported in MTBLS849.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'><span class='label label-success'>MTBLS826</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'><span class='label label-success'>MTBLS839</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'><span class='label label-success'>MTBLS849</span></a>
Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> Method development is reported in the current study MTBLS826.</br> The effect of salt and water on metabolite detection is reported in MTBLS839.</br> Cell culture sampling is reported in MTBLS843.</br> Environmental sampling is reported in MTBLS844.</br> Method quantification is reported in MTBLS848.</br> Solid phase extraction is reported in MTBLS849.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'><span class='label label-success'>MTBLS839</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'><span class='label label-success'>MTBLS848</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'><span class='label label-success'>MTBLS849</span></a>
Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> The effect of salt and water on metabolite detection is reported in the current study MTBLS839.</br> Method development is reported in MTBLS826.</br> Cell culture sampling is reported MTBLS843.</br> Environmental sampling is reported MTBLS844.</br> Method quantification is reported in MTBLS848.</br> Solid phase extraction is reported in MTBLS849.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'><span class='label label-success'>MTBLS826</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'><span class='label label-success'>MTBLS848</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'><span class='label label-success'>MTBLS849</span></a>
Project description:16s RNA gene sequencing data from seawater, bed sediment and steel corrosion samples from Shoreham Harbour, UK, collected to allow bacterial species comparisons between microbially influenced corrosion, the surrounding seawater, and the sea bed sediment at the seafloor and 50cm depth below seafloor.
Project description:<p>The data are dissolved organic compounds from three stations sampled in the western Atlantic Ocean in 2013. Seawater samples spanning from the surface ocean to the seafloor were collected, filtered, acidified, and extracted using solid phase extraction with Bond Elut PPL cartridges. The extracts were separated using reversed phase chromatography and analyzed with ultrahigh resolution mass spectrometry in negative ion mode. The resulting data are features, or a unique combination of mass-to -charge values and retention time. </p>
Project description:Development of a modified solid phase extraction (SPE) approach for improved peptide yield and analysis sensitivity with LC-MS based peptidomics of urine.