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GNPS - LC-MS based phytochemical profiling towards the identification of antioxidant markers in some endemic Aloe species from Mascarene Islands

ABSTRACT: Aloe plant species have been used for centuries in traditional medicine and reported to be an important source of natural products. However, despite the large number of species within the Aloe genus, only a few of them have been investigated chemotaxonomically. A Molecular Network approach was used to highlight the different chemical classes characterizing the leaves of five Aloe species: Aloe macra, Aloe vera, Aloe tormentorii, Aloe ferox and Aloe purpurea. Aloe macra, A. tormentorii and A. purpurea are endemic from the Mascarene Islands comprising Reunion, Mauritius and Rodrigues. UHPLC-MS/MS analysis followed by a dereplication process allowed the characterization of 93 metabolites. The newly developed MolNotator algorithm was used as a tool for molecular networking and allowed a better exploration of the Aloe metabolome chemodiversity. The five species appeared to be rich in polyphenols (anthracene derivatives, flavonoids, phenolic acids). Therefore, total phenolic content and antioxidant activity of the five species were evaluated, and a DPPH-On-Line-HPLC assay was used to determine the metabolites responsible for the radical scavenging activity. The use of computational tools allowed a better description of the chemotaxonomy of five Aloe species, which showed differences in their metabolite composition, both qualitative and quantitative. Moreover, the molecular network approach allowed the identification of metabolites responsible for the antioxidant activity.

INSTRUMENT(S): impact II

ORGANISM(S): Aloe Macra (ncbitaxon:212387) Aloe Ferox (ncbitaxon:117798) Aloe Tormentorii (ncbitaxon:1908395) Aloe Purpurea (ncbitaxon:49726) Aloe Vera (ncbitaxon:34199)

SUBMITTER: Elnur Garayev

PROVIDER: MSV000089679 | GNPS | Fri Jun 17 05:29:00 BST 2022

REPOSITORIES: GNPS

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Project description:The conservation of plant genetic resources (PGRs) is contingent on their current or potential use, so extending the knowledge about them is crucial for their valorization. The valorization and conservation of wild fruit PGRs are critical to ensure the maintenance of genetic and cultural heritage and to promote and develop of new perspectives on resource use. New strategies for characterizing PGRs are needed, and the omics approach can provide profound information that is still largely unknown. The strawberry tree (Arbutus unedo L.) is an underutilized, drought and fire-resistant species distributed in the Mediterranean area and its berries have large ethnobotanical use but were not usually consumed as fresh fruits. Although their phenolic profile and antioxidant capacity are known, they are not well characterised, particularly from a proteomic perspective. The aim of this work is the characterisation from a molecular viewpoint of two ecotypes of A. unedo (Campania and Sicily) to valorise a less known wild fruit PGR to encourage its preservation. Samples were collected from two different geographical areas to assess whether different geographical conditions could influence the characteristics of leaves and fruits at the three stages of ripening (green, veraison, red). Proteomic analysis identified 904 proteins, of which 122 showed significance along the ripening process. Some of these differentially abundant proteins, such as chalcone synthase, show a marked increase during ripening. From a proteomic perspective, there are no differences between the fruits from the two regions compared by the ripening stage. However, the pedoclimatic metabolic imprinting allowed the observation of good diversity in the metabolomic profiles between the two ecotypes, especially for anthocyanins and catechins, generally more abundant in Sicilian fruit, or the phenolic compounds more abundant in Campania fruit. Multi-omic characterisation enhanced the knowledge on a wild fruit plant species which shows specific adaptations and responses to the environment to be considered when addressing the issue of local agrobiodiversity.

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GNPS - LC-MS based phytochemical profiling towards the identification of antioxidant markers in some endemic Aloe species from Mascarene Islands

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