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Protein ID of ϕRP31

ABSTRACT: Jumbo phages ϕRP31/ϕRP12 infecting Ralstonia solanacearum were isolated in Thailand. Virion proteins of ϕRP31 separated by SDS-PAGE were visualized with Coomassie Brilliant Blue. The protein bands were excised from the gel, digested with trypsin, and analyzed by liquid chromatography-tandem mass spectrometry (LTQ Orbitrap XL). Assignment of tandem mass spectrometry data to tryptic peptides encoded by phage open reading frames was completed using an established procedure (Ahmad et al., 2014). Based on these genomic similarities, proteomic tree and gene phylogenies, we propose that ϕRP12 and ϕRP31 are new members of the group of ϕKZ-related phages.

ORGANISM(S): Phage

SUBMITTER: Miyako Nakano

PROVIDER: PXD006355 | JPOST Repository | Sun May 21 00:00:00 BST 2017

REPOSITORIES: jPOST

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			Action	DRS
	161220_02_260kDa.RAW	Raw
	161220_03_180kDa.RAW	Raw
	161220_05_120kDa.RAW	Raw
	161220_06_110kDa.RAW	Raw
	161220_07_100kDa_80kDa.RAW	Raw

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Publications

Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase.

Matsui Takeru T Yoshikawa Genki G Mihara Tomoko T Chatchawankanphanich Orawan O Kawasaki Takeru T Nakano Miyako M Fujie Makoto M Ogata Hiroyuki H Yamada Takashi T

Frontiers in microbiology 20170613

<i>Ralstonia solanacearum</i> phages ΦRP12 and ΦRP31 are jumbo phages isolated in Thailand. Here we show that they exhibit similar virion morphology, genome organization and host range. Genome comparisons as well as phylogenetic and proteomic tree analyses support that they belong to the group of ΦKZ-related phages, with their closest relatives being <i>R. solanacearum</i> phages ΦRSL2 and ΦRSF1. Compared with ΦRSL2 and ΦRSF1, ΦRP12 and ΦRP31 possess larger genomes (ca. 280 kbp, 25% larger). The ...[more]

PMID: 28659872

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