Dataset Information

K562 Chemical arrest cell cycle phase fractions 2D-MS-CETSA

ABSTRACT: K562 cells were synchronized by double thymidine block(DTB) based chemical arrest scheme into G1/S, S and Prometaphase(PM) fractions. Three biological replicates were collected on different days, and assembled into a 2D-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC,54ºC and 57ºC), (Figure 1A, Methods section of the associated paper), with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on Mass Spectrometer. The labeling order of samples in the TMT10 channels is as follows: 37C/47C/50C/52C/54C/57C samples: Biorep1_G1S(126), Biorep1_S(127N), Biorep1_PM(127C), Biorep2_G1S(128N), Biorep2_S(128C), Biorep2_PM(129N), Biorep3_G1S(129C), Biorep3_S(130N), Biorep3_PM(130C), mixed_control(131)

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Pär Nordlund

PROVIDER: PXD009146 | JPOST Repository | Tue May 08 00:00:00 BST 2018

REPOSITORIES: jPOST

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Dataset's files

Source:

			Action	DRS
	20170502_LY_K562_CellCycle_ChemArrest_37C_3bio_rep1_1.raw	Raw
	20170502_LY_K562_CellCycle_ChemArrest_37C_3bio_rep1_2.raw	Raw
	20170502_LY_K562_CellCycle_ChemArrest_37C_3bio_rep1_3.raw	Raw
	20170502_LY_K562_CellCycle_ChemArrest_37C_3bio_rep1_4.raw	Raw
	20170502_LY_K562_CellCycle_ChemArrest_37C_3bio_rep1_5.raw	Raw

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Publications

Modulation of Protein-Interaction States through the Cell Cycle.

Dai Lingyun L Zhao Tianyun T Bisteau Xavier X Sun Wendi W Prabhu Nayana N Lim Yan Ting YT Sobota Radoslaw M RM Kaldis Philipp P Nordlund Pär P

Cell 20180426 6

Global profiling of protein expression through the cell cycle has revealed subsets of periodically expressed proteins. However, expression levels alone only give a partial view of the biochemical processes determining cellular events. Using a proteome-wide implementation of the cellular thermal shift assay (CETSA) to study specific cell-cycle phases, we uncover changes of interaction states for more than 750 proteins during the cell cycle. Notably, many protein complexes are modulated in specifi ...[more]

PMID: 29706543

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