Proteomics

Dataset Information

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IMPRINTS-CETSA, FUDR and FUR in MCF7 cells, 2h and 12h incubation

ABSTRACT: MCF7 cells were treated with floxuridine (FUDR), fluorouridine (FUR), or vehicle and assembled into an IMPRINTS-CETSA scheme with 6 different heating temperatures (37ºC, 47ºC, 50ºC, 52ºC, 54ºC, and 57ºC) with each set of samples heated at one temperature included in the same isobaric TMT10 set and measured at the same time on the mass spectrometer. This entry contains two IMPRINTS-CETSA datasets: 1. MCF7 incubated with Vehicle/FUDR/FUR for 2 hours 2. MCF7 incubated with Vehicle/FUDR/FUR for 12 hours The labelling order of samples in the TMT10 channels for both datasets was as follows: 37C/47C/50C/52C/54C/57C samples: Biorep1_Vehicle (126), Biorep1_FUDR (127N), Biorep1_FUR (127C), Biorep2_Vehicle (128N), Biorep2_FUDR (128C), Biorep2_FUR (129N), Biorep3_Vehicle (129C), Biorep3_FUDR (130N), Biorep3_FUR (130C), mixed_control (131). For additional details see the methods section of the associated publication.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Pär Nordlund 

PROVIDER: PXD026471 | JPOST Repository | Thu Jul 15 00:00:00 BST 2021

REPOSITORIES: jPOST

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Dataset's files

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Action DRS
MCF7_FUDR_FUR_12h_37C_Proteins.txt Txt
MCF7_FUDR_FUR_12h_47C_Proteins.txt Txt
MCF7_FUDR_FUR_12h_50C_Proteins.txt Txt
MCF7_FUDR_FUR_12h_52C_Proteins.txt Txt
MCF7_FUDR_FUR_12h_54C_Proteins.txt Txt
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Publications

CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity.

Liang Ying Yu YY   Bacanu Smaranda S   Sreekumar Lekshmy L   Ramos Anderson Daniel AD   Dai Lingyun L   Michaelis Martin M   Cinatl Jindrich J   Seki Takahiro T   Cao Yihai Y   Coffill Cynthia R CR   Lane David P DP   Prabhu Nayana N   Nordlund Pär P  

Cell chemical biology 20210716 4

The optimal use of many cancer drugs is hampered by a lack of detailed understanding of their mechanism of action (MoA). Here, we apply a high-resolution implementation of the proteome-wide cellular thermal shift assay (CETSA) to follow protein interaction changes induced by the antimetabolite 5-fluorouracil (5-FU) and related nucleosides. We confirm anticipated effects on the known main target, thymidylate synthase (TYMS), and enzymes in pyrimidine metabolism and DNA damage pathways. However, m  ...[more]

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