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Affinity purification using compound-immobilized beads

ABSTRACT: A drug target was explored by affinity purification using the compound-immobilized beads.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Kazuishi Kubota

PROVIDER: PXD010349 | JPOST Repository | Wed Jul 10 00:00:00 BST 2019

REPOSITORIES: jPOST

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Publications

Perturbation-Based Proteomic Correlation Profiling as a Target Deconvolution Methodology.

Ohki Yu Y Sakurai Hidetaka H Hoshino Madoka M Terashima Hideki H Shimizu Hiroki H Ishikawa Tomio T Ogiyama Tomoko T Muramatsu Yasunori Y Nakanishi Toshiyuki T Miyazaki Shojiro S Tsuruoka Hiroyuki H Kobayashi Hideki H Kubota Kazuishi K

Cell chemical biology 20181115 1

Molecular target identification of small molecules, so-called target deconvolution, is a major obstacle to phenotype-based drug discovery. Here, we developed an approach called perturbation-based proteomic correlation profiling (PPCP) utilizing the correlation between protein quantity and binding activity of compounds under cellular perturbation by gene silencing and successfully identified lanosterol synthase as a molecular target of TGF-β pathway inhibitor. This PPCP concept was extended to th ...[more]

PMID: 30449674

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