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Proteome analysis of brainstem organoids from human pluripotent stem cells

ABSTRACT: Brain organoids derived from human pluripotent stem cells recapitulate the course of human brain development. We applied proteomic analysis to human brainstem organoids which were developed by a novel method.

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Shushi Nagamori

PROVIDER: PXD016807 | JPOST Repository | Fri May 01 00:00:00 BST 2020

REPOSITORIES: jPOST

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			Action	DRS
	03_ipscells_F0.raw	Raw
	04_ipscells_F1.raw	Raw
	05_ipscells_F2.raw	Raw
	06_ipscells_F3.raw	Raw
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Brainstem Organoids From Human Pluripotent Stem Cells.

Eura Nobuyuki N Matsui Takeshi K TK Luginbühl Joachim J Matsubayashi Masaya M Nanaura Hitoki H Shiota Tomo T Kinugawa Kaoru K Iguchi Naohiko N Kiriyama Takao T Zheng Canbin C Kouno Tsukasa T Lan Yan Jun YJ Kongpracha Pornparn P Wiriyasermkul Pattama P Sakaguchi Yoshihiko M YM Nagata Riko R Komeda Tomoya T Morikawa Naritaka N Kitayoshi Fumika F Jong Miyong M Kobashigawa Shinko S Nakanishi Mari M Hasegawa Masatoshi M Saito Yasuhiko Y Shiromizu Takashi T Nishimura Yuhei Y Kasai Takahiko T Takeda Maiko M Kobayashi Hiroshi H Inagaki Yusuke Y Tanaka Yasuhito Y Makinodan Manabu M Kishimoto Toshifumi T Kuniyasu Hiroki H Nagamori Shushi S Muotri Alysson R AR Shin Jay W JW Sugie Kazuma K Mori Eiichiro E

Frontiers in neuroscience 20200626

The brainstem is a posterior region of the brain, composed of three parts, midbrain, pons, and medulla oblongata. It is critical in controlling heartbeat, blood pressure, and respiration, all of which are life-sustaining functions, and therefore, damages to or disorders of the brainstem can be lethal. Brain organoids derived from human pluripotent stem cells (hPSCs) recapitulate the course of human brain development and are expected to be useful for medical research on central nervous system dis ...[more]

PMID: 32670003

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Brainstem organoids from human pluripotent stem cells contain neural crest population

Project description:Brainstem controls heartbeat, blood pressure and respiration which are vital for life, therefore, damage to brainstem can be lethal. Brain organoids derived from human pluripotent stem cells recapitulate the course of human brain development and are expected to be useful for medical research on central nervous system disorders. However, existing organoid models have limitations, hampering the elucidation of diseases affecting specific components of the brain. Here, we developed a method to generate human brainstem organoids (hBSOs), containing neural crest stem cells as well as midbrain/hindbrain progenitors, noradrenergic and cholinergic neurons, and dopaminergic neurons, demonstrated by specific electrophysiological signatures. Single-cell RNA-sequence analysis, together with proteomics and electrophysiology further revealed that the cellular population in the organoids is similar to that of the human brainstem and neural crest, which makes it possible to make use of the hBSOs for modeling and grafting for transplantation.

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Metabolomic Profiling of Human Pluripotent Stem Cell Differentiation into Lung Progenitors

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An Approach to Measuring Protein Turnover in Human Induced Pluripotent Stem Cell Organoids by Mass Spectrometry

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