Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MEMA HMEC240L Staining Set 1

ABSTRACT: HMEC240L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), phalloidin (actin), CellMask (cytoplasm) and MitoTracker (metabolic activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

PROVIDER: LDS-1435 | LINCS | 2016-11-10

REPOSITORIES: LINCS

ACCESS DATA

Json Xml

Shared Molecules

Only show the datasets with similarity scores above: 0.5

Threshold

0.5

Similar Datasets

MEMA MCF10A Staining Set 1

Project description:MCF10A cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), phalloidin (actin), CellMask (cytoplasm) and MitoTracker (metabolic activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1443 | LINCS

MEMA HMEC122L Staining Set 1

Project description:HMEC122L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), phalloidin (actin), CellMask (cytoplasm) and MitoTracker (metabolic activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1427 | LINCS

MEMA MCF10A Staining Set 3

Project description:MCF10A cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), CellMask (cytoplasm) KRT5 (basal lineage) and KRT19 (luminal lineage). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1447 | LINCS

MEMA HMEC240L Staining Set 4

Project description:HMEC240L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(basal lineage), KRT19(luminal lineage) and EdU(Active S phase). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1439 | LINCS

MEMA HMEC122L Staining Set 4

Project description:HMEC122L cells were grown in standard 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(basal lineage), KRT19(luminal lineage) and EdU(Active S phase). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1431 | LINCS

Deep phenotyping of peripheral mononuclear blood cells in health and disease

Project description:Peripheral blood mononuclear cells (PBMCs) were isolated from healthy controls and patients with various diseases.. Cells were stained with CD45 and sample tags for identification purposes. CD45+ live cells were enriched through flow sorting and samples were pooled. Samples were then stained with 30 AbSeq antibodies and loaded onto 4 cartridges to account for interplate differences. Pooled sequencing libraries were then sequenced on NovaSeq 6000 (Illumina) using a S2 Reagent Kit v1.5 (200 cycles).

2025-01-06 | GSE280319 | GEO

Transcriptome analysis for differentiated gene expression analysis of TU2218, Vactosertib, and Ramucirumab on VEGF-induced endothelial cell anergy condition

Project description:Transcriptome analysis of HUVECs treated with TGFβRI or VEGFR2 inhibitors on endothelial cell anergy condition In this study, the dual inhibitory mechanism of TU2218 was identified using an in vitro analysis mimicking the tumor microenvironment and its anti-tumor effects were analyzed using in vivo mouse syngeneic tumor models. TU2218 directly normalized the activity of damaged T lymphocytes and natural killer cells from TGFβ and suppressed the activity and viability of regulatory T cells. The anergy of endothelial cells induced by VEGF stimulation was completely ameliorated by TU2218, an effect not observed with vactosertib, which only inhibits TGFβ signal.

2023-12-27 | GSE239760 | GEO

Feed-forward Inhibition of Androgen Receptor Activity by Glucocorticoid Action in Human Adipocytes

Project description:We compared transcriptomes of terminally differentiated mouse 3T3-L1 adipocytes [Series GSE14004] and human adipocytes to identify cell-specific differences. Gene expression and high content analysis (HCA) data identified the androgen receptor (AR) as both expressed and functional, exclusively during early human adipocyte differentiation. We used HCA to determine mechanisms of AR regulation and activity in human adipocytes. We found that AR is regulated in a feed-forward fashion by glucocorticoids.

2012-07-13 | GSE39342 | GEO

MEMA HCC1954 Staining Set 100 (Lapatinib treatment)

Project description:HCC1954 cells were grown in media with Lapatinib in 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(Basal lineage), KRT19 (luminal lineage) and EdU (S phase activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1477 | LINCS

MEMA AU565 Staining Set 100 (Lapatinib treatment)

Project description:AU565 cells were grown in media with Lapatinib in 8 well MEMA for 72h and then fixed using 2% paraformaldehyde. Cells were stained with DAPI (nuclei), KRT5(Basal lineage), KRT19 (luminal lineage) and EdU (S phase activity). The cells were imaged on a Nikon HCA microscopy system, segmented with Cell Profiler, normalized using RUV and lowess using the spatial residuals as controls, and analyzed to identify microenvironment conditions that altered phenotypes of interest.

2016-11-10 | LDS-1469 | LINCS

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data