Proteomics

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Feng_P120_VS1_BRPF3_HBO1_complex

ABSTRACT: This submission contains the mass spectrometry files for the manuscript by Feng et al. that describes the characterization of a protein complex containing BRPF3 and HBO1 in the regulation of DNA replication origins. This submission contains 3 IDA files for the following baits: empty 3XFLAG control, BRPF1-3XFLAG-HA and BRPF3-3XFLAG-HA.

OTHER RELATED OMICS DATASETS IN: PRJNA269250

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Anne-Claude Gingras 

PROVIDER: MSV000078943 | MassIVE | Wed Nov 19 14:46:00 GMT 2014

REPOSITORIES: MassIVE

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Publications

During DNA replication, thousands of replication origins are activated across the genome. Chromatin architecture contributes to origin specification and usage, yet it remains unclear which chromatin features impact on DNA replication. Here, we perform a RNAi screen for chromatin regulators implicated in replication control by measuring RPA accumulation upon replication stress. We identify six factors required for normal rates of DNA replication and characterize a function of the bromodomain and  ...[more]

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