Proteomics

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The value of subcellular fractionation for protein identification in a B-cell line (Ramos)

ABSTRACT: Integrated in the Chromosomic-centric Human Proteome Project (C-HPP), a lymphocytic B-cell line (Ramos) was selected as a model of B-cells. We performed a subfractionation strategy (in triplicate) for the extraction of proteins from 4 different fractions (cytoplasm, CYT; organella, ORG; membrane, MB; nucleus, NUC).

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Manuel Fuentes 

PROVIDER: MSV000079936 | MassIVE | Wed Jul 20 00:43:00 BST 2016

SECONDARY ACCESSION(S): PXD001933

REPOSITORIES: MassIVE

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Publications

Integration of Proteomics and Transcriptomics Data Sets for the Analysis of a Lymphoma B-Cell Line in the Context of the Chromosome-Centric Human Proteome Project.

Díez Paula P   Droste Conrad C   Dégano Rosa M RM   González-Muñoz María M   Ibarrola Nieves N   Pérez-Andrés Martín M   Garin-Muga Alba A   Segura Víctor V   Marko-Varga Gyorgy G   LaBaer Joshua J   Orfao Alberto A   Corrales Fernando J FJ   De Las Rivas Javier J   Fuentes Manuel M  

Journal of proteome research 20150805 9

A comprehensive study of the molecular active landscape of human cells can be undertaken to integrate two different but complementary perspectives: transcriptomics, and proteomics. After the genome era, proteomics has emerged as a powerful tool to simultaneously identify and characterize the compendium of thousands of different proteins active in a cell. Thus, the Chromosome-centric Human Proteome Project (C-HPP) is promoting a full characterization of the human proteome combining high-throughpu  ...[more]

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