Dataset Information

The value of subcellular fractionation for protein identification in a B-cell line (Ramos)

ABSTRACT: Integrated in the Chromosomic-centric Human Proteome Project (C-HPP), a lymphocytic B-cell line (Ramos) was selected as a model of B-cells. We performed a subfractionation strategy (in triplicate) for the extraction of proteins from 4 different fractions (cytoplasm, CYT; organella, ORG; membrane, MB; nucleus, NUC).

REANALYSED by: PAe005366PAe005400PAe005388PAe005383

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): B Cell, Ramos Cell

DISEASE(S): Burkitt Lymphoma

SUBMITTER: Paula Díez

LAB HEAD: Manuel Fuentes

PROVIDER: PXD001933 | Pride | 2015-08-04

REPOSITORIES: Pride

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Publications

Integration of Proteomics and Transcriptomics Data Sets for the Analysis of a Lymphoma B-Cell Line in the Context of the Chromosome-Centric Human Proteome Project.

Díez Paula P Droste Conrad C Dégano Rosa M RM González-Muñoz María M Ibarrola Nieves N Pérez-Andrés Martín M Garin-Muga Alba A Segura Víctor V Marko-Varga Gyorgy G LaBaer Joshua J Orfao Alberto A Corrales Fernando J FJ De Las Rivas Javier J Fuentes Manuel M

Journal of proteome research 20150805 9

A comprehensive study of the molecular active landscape of human cells can be undertaken to integrate two different but complementary perspectives: transcriptomics, and proteomics. After the genome era, proteomics has emerged as a powerful tool to simultaneously identify and characterize the compendium of thousands of different proteins active in a cell. Thus, the Chromosome-centric Human Proteome Project (C-HPP) is promoting a full characterization of the human proteome combining high-throughpu ...[more]

PMID: 26216070

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