Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Proteome profiling of microvesicles, exosomes and supernatant from SKOV3

ABSTRACT: Proteome-metabolome profiling of ovarian cancer ascites reveals novel components involved in intercellular communication http://www.mcponline.org/content/early/2014/09/30/mcp.M114.041194.full.pdf+html?sid=78e7a955-fa54-4ded-b296-89f1fb6adbec

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Dr Georgij Arapidi

PROVIDER: MSV000079992 | MassIVE | Fri Jul 22 16:08:00 BST 2016

SECONDARY ACCESSION(S): PXD001402

REPOSITORIES: MassIVE

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Publications

Proteome-metabolome profiling of ovarian cancer ascites reveals novel components involved in intercellular communication.

Shender Victoria O VO Pavlyukov Marat S MS Ziganshin Rustam H RH Arapidi Georgij P GP Kovalchuk Sergey I SI Anikanov Nikolay A NA Altukhov Ilya A IA Alexeev Dmitry G DG Butenko Ivan O IO Shavarda Alexey L AL Khomyakova Elena B EB Evtushenko Evgeniy E Ashrafyan Lev A LA Antonova Irina B IB Kuznetcov Igor N IN Gorbachev Alexey Yu AY Shakhparonov Mikhail I MI Govorun Vadim M VM

Molecular & cellular proteomics : MCP 20140930 12

Ovarian cancer ascites is a native medium for cancer cells that allows investigation of their secretome in a natural environment. This medium is of interest as a promising source of potential biomarkers, and also as a medium for cell-cell communication. The aim of this study was to elucidate specific features of the malignant ascites metabolome and proteome. In order to omit components of the systemic response to ascites formation, we compared malignant ascites with cirrhosis ascites. Metabolome ...[more]

PMID: 25271300

Similar Datasets

Proteome profiling of microvesicles, exosomes and supernatant from SKOV3

Project description:Proteome-metabolome profiling of ovarian cancer ascites reveals novel components involved in intercellular communication http://www.mcponline.org/content/early/2014/09/30/mcp.M114.041194.full.pdf+html?sid=78e7a955-fa54-4ded-b296-89f1fb6adbec

2014-10-23 | PXD001402 | Pride

Proteome profiling of ovarian cancer and cirrhosis ascites

2014-10-23 | PXD001403 | Pride

Proteome profiling of ovarian cancer and cirrhosis ascites

2016-07-19 | MSV000079934 | MassIVE

The Role of Extracellular Spliceosomal snRNAs in Communication of Ovarian Cancer Cells

Project description:Exogenous signals from drug-stressed cancer cells accelerate the proliferation of neighboring tumor cells and promote them to acquire a more aggressive phenotype. We have recently found an exciting phenomenon: drug-stressed cancer cells secrete various components of the spliceosome: proteins and a number of snRNAs. We have observed this phenomenon both in vitro (culture media from cancer cell lines [Pavluykov M. et al.//Cancer Cell, 2018]) and in vivo (ovarian cancer ascites after chemotherapy [Shender V. et al//Mol. Сell. Proteomics, 2014]). The aim of this study was to elucidate the role of secreted spliceosomal snRNAs in intercellular communication. We constructed synthetic U12 and U6atac snRNAs close to the natural structure, including some non-canonical nucleotides imitating post-transcriptional RNA modification. Proteomic analysis of SKOV3 cells transfected with U6atac or U12 snRNA analogs has shown that both exogenous snRNAs lead to an increase of abundance of proteins related to cell cycle regulation and M phase. It has been recently shown that a number of spliceosomal proteins affect regulation of the cell cycle, in particular the M phase (Maslon et al., 2014). Here we demonstrated that not only spliceosomal proteins but also spliceosomal snRNAs (U12 and U6atac) affect activation of mitotic cell cycle genes. This study reveals previously unknown signaling molecules in the microenvironment of ovarian cancer that have potential clinical significance.

2024-05-29 | PXD027950 | Pride

Effect of extracellular spliceosomal snRNAs on the transcriptome of ovarian cancer cells

Project description:Exogenous signals from drug-stressed cancer cells accelerate the proliferation of neighboring tumor cells and promote to acquire a more aggressive phenotype. We have recently found an exciting phenomenon: drug-stressed cancer cells secrete various components of the spliceosome: proteins and a number of snRNAs. We have observed this phenomenon both in vitro (culture media from cancer cell lines [Pavluykov M. et al., 2018]) and in vivo (ovarian cancer ascites after chemotherapy [Shender V. et al., 2014]). The aim of this study was to elucidate the role of secreted spliceosomal snRNAs in intercellular communication. We constructed synthetic U12 and U6atac snRNAs close to the natural structure, including some non-canonical nucleotides imitating post-transcriptional RNA modifications. RNAseq analysis of SKOV3 cells transfected with U6atac or U12 snRNA analogs has shown that both exogenous snRNAs lead to an increase of abundance of proteins related to cell cycle regulation and M phase. It has been recently shown that a number of spliceosomal proteins affect regulation of the cell cycle, in particular the M phase [Maslon et al., 2014]. Here we demonstrated that not only spliceosomal proteins but also spliceosomal snRNAs (U12 and U6atac) affect cell cycle gene expression. This study revealed previously unknown signaling molecules in the microenvironment of ovarian cancer that have potential clinical significance.

2024-04-24 | GSE241912 | GEO

ChIP-seq from liver (ENCSR769JUI)

Project description:Chip-Seq on STL010-human (fragmentation date: 2014-09-15) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-03-12 | GSE96367 | GEO

ChIP-seq from liver (ENCSR628ONI)

Project description:Chip-Seq on STL011-human (fragmentation date: 2014-09-15) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-03-12 | GSE96275 | GEO

ChIP-seq from lung (ENCSR718KRA)

Project description:Chip-Seq on e14.5 lung-mouse (fragmentation date:2014-09-30) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE92008 | GEO

ChIP-seq from limb (ENCSR164BWS)

Project description:Chip-Seq on e14.5 limb-mouse (fragmentation date: 2014-09-30) For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE91542 | GEO

Screening for the key miRNAs of exosomes that promote tumor progression and metastasis

Project description:Intercellular communication is crucial for breast cancer progression and metastasis. Yet, the role of cancer-derived exosomes and their specific miRNAs cargo in mediating intercellular communications needed to be further elucidated. MiRNAs control gene expression post-transcriptionally and are selectively packaged into cancer-derived exosomes. Therefore, we hypothesized that miRNAs in cancer-derived exosomes were most likely the essential components of intercellular communication. To identify the key miRNAs, we chose metastatic MDA-MB-231 and non-metastatic MCF-7 for comparison. The miRNAs profiling of exosomes derived from these two kinds of cells as well as the endogenous miRNAs profiling of the cells were conducted by microarrays.

2020-07-17 | GSE154054 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data