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Omics score: 2

Human milk whey proteins treated Caco-2 cells

ABSTRACT: Human milk whey proteins facilitate the development of enterocyte-like Caco-2 cells over time.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Qiang Zhang

PROVIDER: MSV000080811 | MassIVE | Thu Mar 30 16:22:00 BST 2017

SECONDARY ACCESSION(S): PXD002121

REPOSITORIES: MassIVE

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Publications

Sensing Small Changes in Protein Abundance: Stimulation of Caco-2 Cells by Human Whey Proteins.

Cundiff Judy K JK McConnell Elizabeth J EJ Lohe Kimberly J KJ Maria Sarah D SD McMahon Robert J RJ Zhang Qiang Q

Journal of proteome research 20151215 1

Mass spectrometry (MS)-based proteomic approaches have largely facilitated our systemic understanding of cellular processes and biological functions. Cutoffs in protein expression fold changes (FCs) are often arbitrarily determined in MS-based quantification with no demonstrable determination of small magnitude changes in protein expression. Therefore, many biological insights may remain veiled due to high FC cutoffs. Herein, we employ the intestinal epithelial cell (IEC) line Caco-2 as a model ...[more]

PMID: 26586228

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Human milk whey proteins treated Caco-2 cells

Project description:Human milk whey proteins facilitate the development of enterocyte-like Caco-2 cells over time.

2015-11-23 | PXD002121 | Pride

Discovery-driven target selection from six milk-derived ingredients for consensus detection of casein and whey milk allergens

Project description:Milk is one among the most common food allergens and the proteins representing caseins and whey fractions of milk have been characterized as the major triggers for allergic responses in susceptible individuals. Milk or milk-derived ingredients- varying in their casein and whey protein content and composition- are present as an ingredient in many foods. This project was designed to identify candidate target peptides for casein and whey fractions of milk which can be used for milk allergen detection from foods irrespective of the type of ingredients used in them.

2020-06-05 | PXD016429 | Pride

Differences in the bovine milk whey proteome between early pregnancy and the estrous cycle

Project description:As current bovine pregnancy detection methods are not reliable until at least day 28 post artificial insemination (AI), commercial interest exists for the discovery of novel biomarkers of pregnancy which could reliably detect pregnancy status at or before day 21 of pregnancy. This would allow producers the opportunity to rebreed at the next oestrus event. Therefore, the objective of the present study was to use liquid chromatography tandem mass spectrometry (LC-MS/MS) to perform a global, label-free, proteomics study on (i) whole milk whey and (ii) extracellular vesicle (EV) enriched milk whey samples, from day 21 of pregnancy and day 21 of the oestrous cycle, in order to identify potential protein biomarkers of early pregnancy. The oestrous cycles of 10 dairy cows were synchronised, they went through one (control) oestrous cycle and these cows were artificially inseminated during the following oestrous. These cows were confirmed pregnant by ultrasound scanning. Milk whey samples were collected on day 21 of the oestrous cycle and on day 21 post AI. A Q-Exactive was used to analyse whole milk whey samples and EV enriched milk whey samples by LC-MS/MS and subsequent analyses of the label-free quantitative data was performed in MaxQuant and Perseus. Four proteins (APOB, SPADH1, PLIN2 and LPO) were differentially expressed between the proteomes of whole milk whey from day 21 of pregnancy and day 21 of the oestrous cycle (P<0.05). Ten proteins (PIGR, PGD, QSOX1, MUC1, SRPRA, MD2, GAPDH, FOLR1, GPRC5B and HHIPL2) were differentially expressed between the proteomes of milk whey from day 21 of pregnancy and day 21 of the oestrous cycle (P<0.05). These proteins are potential milk whey biomarkers of early pregnancy; however, the small fold change differences in their abundance between samples obtained during early pregnancy and during oestrous will restrict their use in a pregnancy diagnosis test.

2018-04-23 | PXD007927 | Pride

Characterisation and comparison site-specific N-glycosylation of whey proteins from donkey and human colostrum and mature milk using glycoproteomics

Project description:Although the composition of donkey milk is similar to that of human milk, systematic comparisons of the site-specific N-glycosylation patterns of their whey proteins are lacking. In this study, hydrophilic interaction chromatography-based enrichment of intact N-glycopeptides, coupled with a site-specific glycoproteomics strategy, was used to systematically characterise whey N-glycoproteins in donkey colostrum (DC), donkey mature milk (DM), human colostrum (HC), and human mature milk (HM) for the first time. We identified 628, 347, 868, and 425 site-specific N-glycans mapped to 135, 67, 113, and 60 glycoproteins in DC, DM, HC, and HM, respectively. Bioinformatic analysis revealed the potential biological effects of N-glycosylation modifications on the whey proteins themselves. Our findings elucidated the composition of donkey and human milk whey N-glycoproteins and their potential structure-activity relationships and provided guidance for the production of specific functional donkey milk products and the development of donkey milk-based infant formulas.

2024-03-19 | PXD050759 |

Expression profiles of microRNA in bovine raw milk exosome and supernatant without exosome [miRNA array]

Project description:We have reported that microRNAs are present in human, bovine, and rat milk whey. Milk whey miRNAs were resistant to acidic condition and to RNase. Thus, milk miRNAs were thought to be present packaged into membrane vesicles like exosome. However, body fluid miRNAs have been reported that there are in different forms. To clarify which miRNAs species are exist in exosome and which species are exist in another form, we used bovine raw milk and purified total RNA from exosome fraction and ultracentrifugated supernatant fraction, and analyzed by miRNA microarray.

2015-03-09 | GSE61976 | GEO

Expression profiles of microRNA in bovine raw milk exosome and supernatant without exosome [mRNA array]

2015-03-09 | GSE61977 | GEO

Proteomic changes of whey proteins after heating at different temperatures

Project description:This study was conducted in order to relatively quantify the changes in the native protein profile of whey after heating milk at 65 °C, 70°C, 75°C, 80°C, 85°C for 30 min

2020-01-27 | PXD016436 | Pride

Comparative proteomics of whey proteins: new insights into quantitative differences between bovine, goat and camel species

Project description:Whey proteins are the leading proteins class in milk and play an essential role in the immune defense of neonatal mammals. The aim of this study was to analyze whey proteins in bovine, goat and camel milk by label free proteomics techniques. Finally, 840 proteins were identified, which considerably increasing the number of whey proteins identified in these species. The results of the PCA revealed significant differences in whey proteome patterns between bovine, goat and camel milk. Proteins such as PAEP, CST3, SERPING1, CTSB and GLG1 play an important role as markers in the classification of bovine, goat and camel milk. Statistical analysis showed that the relative abundances of many whey proteins such as ALB, LALBA, LTF and LPO were significantly different among different species. GO and KEGG functional analysis have shown that while the distribution of biological functions involved in whey proteins was relatively similar across species, they differed in terms of protein quantity. These data shed light on the quantitative differences and potential physiological functions of whey proteins across species, and may point the way to the production of specific functional whey proteins.

2022-10-29 | PXD037828 |

Sheep milk transcriptome

Project description:This study presents a dynamic characterization of the sheep milk transcriptome aiming at achieving a better understanding of the sheep lactating mammary gland. Transcriptome sequencing (RNA-seq) was performed on total RNA extracted from milk somatic cells from ewes on days 10, 50, 120 and 150 after lambing. The experiment was performed in Spanish Churra and Assaf breeds, which differ in their milk production traits. Nearly 67% of the annotated genes in the reference genome (Oar_v3.1) were expressed in ovine milk somatic cells. For the two breeds and across the four lactation stages studied, the most highly expressed genes encoded caseins and whey proteins. We detected differentially expressed genes (DEGs) across lactation points, with the largest differences being found, between day 10 and day 150. Upregulated GO terms at late lactation stages were linked mainly to developmental processes linked to extracellular matrix remodeling. A total of 256 annotated DEGs were detected in the Assaf and Churra comparison. Some genes selectively upregulated in the Churra breed grouped under the endopeptidase and channel activity GO terms. These genes could be related to the higher cheese yield of this breed. Overall, this study provides the first integrated overview on sheep milk gene expression.

2016-06-07 | GSE74825 | GEO

A Comparative Proteomic Analysis of Whey Protein from Early, Mid and Late Lactation Stages of Indian Indigenous Breed, MalnadGidda (Bosindicus)

Project description:Different lactation stages have marked influence on milk yield, milk constituents and nourishment of the neonates. However, the differential gene expression during different lactation stages in Bosindicus has not been investigated so far. In this study, we carried out high-resolution mass spectrometry-based quantitative proteomics of bovine whey at early, mid and late lactation stages of MalnadGidda (Bosindicus) cows. Using TMT-based quantitative proteomics, we compared the bovine whey proteins on progressive lactation stages of Indian breed, MalnadGidda(Bosindicus). LC-MS/MS analysis of whey peptides from early, mid and late lactation stages resulted in the generation of 420,092 MS/MS spectra and 50,800 peptide spectrum matches, which led to the identification of 4,450 peptides corresponding to 725 proteins. Out of which, 440 proteins were differentially expressed (≥1.5-fold). Gene Ontology studies showed that proteins that regulatemilk composition and mammary growth associated proteins are abundantly expressed during peak lactation stages. Whereas, proteins related to pregnancy and mammary involution are expressed high in late and mid lactation stages indicating the physiological changes in the maternal system of bovine during drying period. Detection of progestagen associated endometrial protein; an immune protein seen in the fetomaternal interface and other pregnancy associated proteins at mid lactation suggest a candidate biomarker for the early pregnancy diagnosis. These results are overlapping with the previous findings addressed in milk from exotic breeds. We strongly believe that this preliminary investigation on differential proteome in milk whey over the course of lactation of indigenous cattle could answer many unsolved questions in lactation biology.

2018-02-16 | PXD007655 | Pride

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